BMRB Entry 4377
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4377
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Title: The Solution Structure of the Type X Cellulose Binding Domain from Pseudomonas xylanase A PubMed: 10653641
Deposition date: 1999-08-17 Original release date: 2000-03-02
Authors: Raghothama, Srinivasarao; Simpson, Peter; Szabo, L; Nagy, T; Gilbert, Harry; Williamson, Mike
Citation: Raghothama, Srinivasarao; Simpson, Peter; Szabo, L; Nagy, T; Gilbert, Harry; Williamson, Mike. "Solution structure of the CBM10 cellulose binding module from Pseudomonas Xylanase A" Biochemistry 39, 978-984 (2000).
Assembly members:
type X cellulose binding domain from Pseudomonas xylanase A, polymer, 75 residues, Formula weight is not available
Natural source: Common Name: P. fluorescens Taxonomy ID: 29436 Superkingdom: Eubacteria Kingdom: not available Genus/species: Pseudomonas fluorescens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
type X cellulose binding domain from Pseudomonas xylanase A: HHHHHHHHHHSSGHIAGRHM
GNQQCNWYGTLYPLCVTTTN
GWGWEDQRSCIARSTCAAQP
APFGIVGSGHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 308 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CBDX | 1 |
Entities:
Entity 1, CBDX 75 residues - Formula weight is not available
| 1 | HIS | HIS | HIS | HIS | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | HIS | ILE | ALA | GLY | ARG | HIS | MET | ||||
| 3 | GLY | ASN | GLN | GLN | CYS | ASN | TRP | TYR | GLY | THR | ||||
| 4 | LEU | TYR | PRO | LEU | CYS | VAL | THR | THR | THR | ASN | ||||
| 5 | GLY | TRP | GLY | TRP | GLU | ASP | GLN | ARG | SER | CYS | ||||
| 6 | ILE | ALA | ARG | SER | THR | CYS | ALA | ALA | GLN | PRO | ||||
| 7 | ALA | PRO | PHE | GLY | ILE | VAL | GLY | SER | GLY | HIS | ||||
| 8 | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_one: type X cellulose binding domain from Pseudomonas xylanase A 1 mM
experimental_conditions_one: pH*: 4.5; temperature: 323 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| Homonuclear | not available | not available | not available |
| TOCSY | not available | not available | not available |
| NOESY | not available | not available | not available |
| DQFC | not available | not available | not available |
| E.COSY | not available | not available | not available |
Software:
FELIX v97.0 -
NMR spectrometers:
- Bruker DRX 500 MHz
- Bruker DRX 600 MHz
Related Database Links:
| PDB |