BMRB Entry 4382
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4382
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Title: 1H, 13C and 15N Assigned Chemical Shifts for a Eukaryotic Rubredoxin from Guillardia theta
Deposition date: 1999-08-25 Original release date: 2000-11-30
Authors: Schweimer, Kristian; Hoffmann, Silke; Wastl, J.; Maier, U.-G.; Roesch, Paul; Sticht, Heinrich
Citation: Schweimer, Kristian; Hoffmann, Silke; Wastl, J.; Maier, U.-G.; Roesch, Paul; Sticht, Heinrich. "Solution Structure of a Zinc Substituted Eukaryotic Rubredoxin from the Cryptomonad Algal Guillardia theta" Protein Sci. 9, 1474-1486 (2000).
Assembly members:
Guillardia Theta Rubredoxin, polymer, 70 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Guillardia theta Taxonomy ID: 55529 Superkingdom: Eukaryota Kingdom: not available Genus/species: Guillardia theta
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
Guillardia Theta Rubredoxin: MEIDEGKYECEACGYIYEPE
KGDKFAGIPPGTPFVDLSDS
FMCPACRSPKNQFKSIKKVI
AGFAENQKYG
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 246 |
15N chemical shifts | 68 |
1H chemical shifts | 435 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | rubredoxin | 1 |
2 | zinc | 2 |
Entities:
Entity 1, rubredoxin 70 residues - Formula weight is not available
1 | MET | GLU | ILE | ASP | GLU | GLY | LYS | TYR | GLU | CYS | |
2 | GLU | ALA | CYS | GLY | TYR | ILE | TYR | GLU | PRO | GLU | |
3 | LYS | GLY | ASP | LYS | PHE | ALA | GLY | ILE | PRO | PRO | |
4 | GLY | THR | PRO | PHE | VAL | ASP | LEU | SER | ASP | SER | |
5 | PHE | MET | CYS | PRO | ALA | CYS | ARG | SER | PRO | LYS | |
6 | ASN | GLN | PHE | LYS | SER | ILE | LYS | LYS | VAL | ILE | |
7 | ALA | GLY | PHE | ALA | GLU | ASN | GLN | LYS | TYR | GLY |
Entity 2, zinc - Zn - 65.409 Da.
1 | ZN |
Samples:
sample_1: Guillardia Theta Rubredoxin, [U-95% 13C; U-95% 15N], 2.6 mM
Ex-cond_1: pH: 6.4; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1H-1H NOESY | sample_1 | not available | Ex-cond_1 |
1H-1H TOCSY | sample_1 | not available | Ex-cond_1 |
1H-15N HSQC | sample_1 | not available | Ex-cond_1 |
1H-13C CT-HSQC | sample_1 | not available | Ex-cond_1 |
15N-edited NOESY(3D) | sample_1 | not available | Ex-cond_1 |
13C-edited NOESY(3D) | sample_1 | not available | Ex-cond_1 |
HNCO | sample_1 | not available | Ex-cond_1 |
HNCA | sample_1 | not available | Ex-cond_1 |
HNCACB | sample_1 | not available | Ex-cond_1 |
CBCA(CO)NH | sample_1 | not available | Ex-cond_1 |
HBHA(CO)NH | sample_1 | not available | Ex-cond_1 |
HNHA | sample_1 | not available | Ex-cond_1 |
HCCH-COSY | sample_1 | not available | Ex-cond_1 |
Software:
NMRview v4.0.1 - peak picking and visualization of the NMR data
Home written Software - In-house developed software for data processing and automatic backbone assignment
NMR spectrometers:
- Bruker DRX 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts