BMRB Entry 4421
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4421
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Title: 13C,15N and 1H Assigned Chemical Shifts for PhoB DNA-binding Domain.
Deposition date: 1999-09-26 Original release date: 2001-05-01
Authors: Okamura, Hideyasu; Hanaoka, Shingo; Nagadoi, Aritaka; Makino, Kozo; Nishimura, Yoshihumi
Citation: Okamura, Hideyasu; Hanaoka, Shingo; Nagdoi, Aritaka; Makino, Kozo; Nishimura, Yoshihumi. "13C, 15N and 1H Assigned Chemical Shifts for PhoB DNA-binding Domain." The BMRB entry is the only known published source for the data..
Assembly members:
PHOSPHATE REGULON TRANSCRIPTIONAL REGULATORY PROTEIN PHOB, polymer, 104 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
PHOSPHATE REGULON TRANSCRIPTIONAL REGULATORY PROTEIN PHOB: MAVEEVIEMQGLSLDPTSHR
VMAGEEPLEMGPTEFKLLHF
FMTHPERVYSREQLLNHVWG
TNVYVEDRTVDVHIRRLRKA
LEPGGHDRMVQTVRGTGYRF
STRF
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 316 |
| 15N chemical shifts | 103 |
| 1H chemical shifts | 733 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PhoB DNA-binding domain | 1 |
Entities:
Entity 1, PhoB DNA-binding domain 104 residues - Formula weight is not available
| 1 | MET | ALA | VAL | GLU | GLU | VAL | ILE | GLU | MET | GLN | ||||
| 2 | GLY | LEU | SER | LEU | ASP | PRO | THR | SER | HIS | ARG | ||||
| 3 | VAL | MET | ALA | GLY | GLU | GLU | PRO | LEU | GLU | MET | ||||
| 4 | GLY | PRO | THR | GLU | PHE | LYS | LEU | LEU | HIS | PHE | ||||
| 5 | PHE | MET | THR | HIS | PRO | GLU | ARG | VAL | TYR | SER | ||||
| 6 | ARG | GLU | GLN | LEU | LEU | ASN | HIS | VAL | TRP | GLY | ||||
| 7 | THR | ASN | VAL | TYR | VAL | GLU | ASP | ARG | THR | VAL | ||||
| 8 | ASP | VAL | HIS | ILE | ARG | ARG | LEU | ARG | LYS | ALA | ||||
| 9 | LEU | GLU | PRO | GLY | GLY | HIS | ASP | ARG | MET | VAL | ||||
| 10 | GLN | THR | VAL | ARG | GLY | THR | GLY | TYR | ARG | PHE | ||||
| 11 | SER | THR | ARG | PHE |
Samples:
sample_one: PHOSPHATE REGULON TRANSCRIPTIONAL REGULATORY PROTEIN PHOB, {U-95% 13C; U-90% 15N], 1.0 – 2.0 mM
sample_conditions: pH: 6.8 na; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz
Related Database Links:
| PDB | |
| DBJ | BAB33872 BAE76179 BAG75943 BAI23770 BAI29241 |
| EMBL | CAA27659 CAA56927 CAP74933 CAQ30868 CAQ97270 |
| GB | AAA26535 AAB18123 AAC73502 AAG54745 AAL09901 |
| REF | NP_308476 NP_414933 NP_706287 WP_000113916 WP_000113917 |
| SP | P0AFJ5 P0AFJ6 P45606 P45607 |
| AlphaFold | P0AFJ5 P0AFJ6 P45606 P45607 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts