BMRB Entry 4424

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4424
MolProbity Validation Chart

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Title:
NMR Structure of the N-terminal Domain of Saccharomyces cerevisiae RNase HI Reveals a Fold with a Strong Resemblance to the N-terminal Domain of Ribosomal Protein L9
Deposition date:
1999-09-29
Original release date:
1999-12-23
Authors:
Evans, S.; Bycroft, M.
Citation:

Citation: Evans, S.; Bycroft, M.. "NMR Structure of the N-terminal Domain of Saccharomyces cerevisiae RNase HI Reveals a Fold with a Strong Resemblance to the N-terminal Domain of Ribosomal Protein L"  J. Mol. Biol. 291, 661-669 (1999).

Assembly members:

Assembly members:
ribonuclease HI, polymer, 63 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ribonuclease HI: GSARQGNFYAVRKGRETGIY NTWNECKNQVDGYGGAIYKK FNSYEQAKSFLGQPNTTSNY GSS

Data sets:
Data typeCount
1H chemical shifts345
13C chemical shifts190
15N chemical shifts58

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RNase HI1

Entities:

Entity 1, RNase HI 63 residues - Formula weight is not available

1   GLYSERALAARGGLNGLYASNPHETYRALA
2   VALARGLYSGLYARGGLUTHRGLYILETYR
3   ASNTHRTRPASNGLUCYSLYSASNGLNVAL
4   ASPGLYTYRGLYGLYALAILETYRLYSLYS
5   PHEASNSERTYRGLUGLNALALYSSERPHE
6   LEUGLYGLNPROASNTHRTHRSERASNTYR
7   GLYSERSER

Samples:

sample_1: ribonuclease HI, [U-13C; U-15N], 1.0 – 3.0 mM

sample_cond_1: pH: 3.6; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1not availablesample_cond_1
COSYsample_1not availablesample_cond_1
TOCSYsample_1not availablesample_cond_1
HBHA(CO)NHsample_1not availablesample_cond_1
CBCA(CO)NHsample_1not availablesample_cond_1
HNCACBsample_1not availablesample_cond_1
HCCH-TOCSYsample_1not availablesample_cond_1
HNHAsample_1not availablesample_cond_1
HNHBsample_1not availablesample_cond_1

Software:

FELIX v2.3 -

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

PDB
DBJ GAA25685
EMBL CAA90205 CAY82065
GB AHY76689 AJP40928 AJS62100 AJS62535 AJS62973
REF NP_013961
SP Q04740
TPG DAA10134
AlphaFold Q04740

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