BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4433

Title: 1H-NMR Spectral Assignment of Second Transmembrane Segments of Human Glycine Receptor   PubMed: 12080117

Deposition date: 1999-10-05 Original release date: 2003-04-23

Authors: Tang, Pei; Zubrzycki, Igor; Hu, Jian; Xu, Yan

Citation: Tang, Pei; Mandal, P.; Xu, Yan. "NMR structures of the Second Transmembrane domain of the human glycine receptor alpha(1) subunit: Model of pore architecture and channel gating."  Biophys. J. 83, 252-262 (2002).

Assembly members:
Neurotransmitter-gated receptor, polymer, 23 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Neurotransmitter-gated receptor: PARVGLGITTVLTMTTQYSG SRA

Data typeCount
1H chemical shifts285

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1GlyR alpha 1 M2 (S267Y)1

Entities:

Entity 1, GlyR alpha 1 M2 (S267Y) 23 residues - Formula weight is not available

1   PROALAARGVALGLYLEUGLYILETHRTHR
2   VALLEUTHRMETTHRTHRGLNTYRSERGLY
3   SERARGALA

Samples:

sample_one: Neurotransmitter-gated receptor 3.1 mM; SDS 677 mM

sample_two: Neurotransmitter-gated receptor 3.1 mM; DPC mM

sample_conditions: pH: 4.8 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-NOESYnot availablenot availablenot available
1H-TOCSYnot availablenot availablenot available

Software:

NMRPipe - Data process

SPARKY - Data analysis

NMR spectrometers:

  • Bruker DMX 750 MHz
  • Bruker DMX 600 MHz