BMRB Entry 4449
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4449
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Intramolecular Dimer Antifreeze Protein RD
Deposition date: 1999-11-02 Original release date: 2000-03-22
Authors: Miura, Kazunori; Ohgiya, Satoru; Hoshino, Tamotsu; Nemoto, Nobuaki; Nitta, Katsutoshi; Tsuda, Sakae
Citation: Miura, Kazunori; Ohgiya, Satoru; Hoshino, Tamotsu; Nemoto, Nobuaki; Nitta, Katsutoshi; Tsuda, Sakae. "Letter to the Editor: Assignments of 1H, 13C, and 15N resonances of intramolecular dimer antifreeze protein RD3" J. Biomol. NMR 16, 273-274 (2000).
Assembly members:
antifreeze protein type III, polymer, 134 residues, Formula weight is not available
Natural source: Common Name: Antarctic eelpout Taxonomy ID: 8195 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Austrolycichthys brachycephalus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pkk223-3UC
Entity Sequences (FASTA):
antifreeze protein type III: NKASVVANQLIPINTALTLI
MMKAEVVTPMGIPAEEIPNL
VGMQVNRAVPLGTTLMPDMV
KNYEDGTTSPGLKSVVANQL
IPINTALTLVMMKAEEVSPK
GIPSEEISKLVGMQVNRAVY
LDQTLMPDMVKNYE
- assigned_chemical_shifts
- coupling_constants
Data type | Count |
13C chemical shifts | 259 |
15N chemical shifts | 140 |
1H chemical shifts | 973 |
coupling constants | 80 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | RD3 | 1 |
Entities:
Entity 1, RD3 134 residues - Formula weight is not available
1 | ASN | LYS | ALA | SER | VAL | VAL | ALA | ASN | GLN | LEU | ||||
2 | ILE | PRO | ILE | ASN | THR | ALA | LEU | THR | LEU | ILE | ||||
3 | MET | MET | LYS | ALA | GLU | VAL | VAL | THR | PRO | MET | ||||
4 | GLY | ILE | PRO | ALA | GLU | GLU | ILE | PRO | ASN | LEU | ||||
5 | VAL | GLY | MET | GLN | VAL | ASN | ARG | ALA | VAL | PRO | ||||
6 | LEU | GLY | THR | THR | LEU | MET | PRO | ASP | MET | VAL | ||||
7 | LYS | ASN | TYR | GLU | ASP | GLY | THR | THR | SER | PRO | ||||
8 | GLY | LEU | LYS | SER | VAL | VAL | ALA | ASN | GLN | LEU | ||||
9 | ILE | PRO | ILE | ASN | THR | ALA | LEU | THR | LEU | VAL | ||||
10 | MET | MET | LYS | ALA | GLU | GLU | VAL | SER | PRO | LYS | ||||
11 | GLY | ILE | PRO | SER | GLU | GLU | ILE | SER | LYS | LEU | ||||
12 | VAL | GLY | MET | GLN | VAL | ASN | ARG | ALA | VAL | TYR | ||||
13 | LEU | ASP | GLN | THR | LEU | MET | PRO | ASP | MET | VAL | ||||
14 | LYS | ASN | TYR | GLU |
Samples:
sample_1: antifreeze protein type III 1.0 mM
sample_2: antifreeze protein type III, [U-90% 15N], 1.0 mM
sample_3: antifreeze protein type III, [U-95% 13C; U-90% 15N], 1.0 mM
Ex-cond_1: ionic strength: 0.025 M; pH: 6.8; temperature: 277 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1H-15N NOESY | not available | not available | Ex-cond_1 |
1H-15N TOCSY | not available | not available | Ex-cond_1 |
HNCA | not available | not available | Ex-cond_1 |
HNCACB | not available | not available | Ex-cond_1 |
CBCA(CO)NH | not available | not available | Ex-cond_1 |
HCCH-TOCSY | not available | not available | Ex-cond_1 |
C(CO)NH | not available | not available | Ex-cond_1 |
HC(CO)NH | not available | not available | Ex-cond_1 |
Software:
NMRPipe v1.6 - data processing
PIPP v4.0.4 - assignment
NMR spectrometers:
- Varian Unity 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts