BMRB Entry 4556
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4556
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Title: 31P NMR analysis of the DNA conformation induced by protein binding:SRY-DNA complexes
Deposition date: 1999-12-11 Original release date: 2000-01-11
Authors: Castagne, Claire; Murphy, Elizabeth; Gronenborn, Angela; Delepierre, Muriel
Citation: Castagne, Claire; Murphy, Elizabeth; Gronenborn, Angela; Delepierre, Muriel. "31P NMR analysis of the DNA conformation induced by protein binding:SRY-DNA complexes" Eur. J. Biochem. ., .-..
Assembly members:
SRY 8mer strand 1, polymer, 8 residues, Formula weight is not available
SRY 8mer strand 2, polymer, 8 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: Eukaryota Kingdom: not available Genus/species: na na
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
SRY 8mer strand 1: GCACAAAC
SRY 8mer strand 2: GTTTGTGC
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 137 |
| 31P chemical shifts | 14 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 8mer strand 1 | 1 |
| 2 | 8mer strand 2 | 2 |
Entities:
Entity 1, 8mer strand 1 8 residues - Formula weight is not available
| 1 | DG | DC | DA | DC | DA | DA | DA | DC |
Entity 2, 8mer strand 2 8 residues - Formula weight is not available
| 1 | DG | DT | DT | DT | DG | DT | DG | DC |
Samples:
SRY-8mer: SRY 8mer strand 1 2.5 mM; SRY 8mer strand 2 2.5 mM
cond1: pH: 7; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H COSY | SRY-8mer | not available | cond1 |
| 1H-1H TOCSY | SRY-8mer | not available | cond1 |
| 1H-1H NOESY | SRY-8mer | not available | cond1 |
| 1H-31P HSQC-TOCSY | SRY-8mer | not available | cond1 |
| 1H-31P HCOSYINV | SRY-8mer | not available | cond1 |
| 31P-31P NOESY Exchange | SRY-8mer | not available | cond1 |
Software:
No software information available
NMR spectrometers:
- Varian Unity 500 MHz