BMRB Entry 4568
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4568
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Title: Native and non-native secondary structure and dynamics in the pH 4 intermediate of apomyoglobin
Deposition date: 2000-01-04 Original release date: 2000-05-25
Authors: Eliezer, David; Chung, John; Dyson, Jane; Wright, Peter
Citation: Eliezer, David; Chung, John; Dyson, Jane; Wright, Peter. "Native and non-native secondary structure and dynamics in the pH 4 intermediate of apomyoglobin" Biochemistry ., .-..
Assembly members:
myoglobin, polymer, 153 residues, Formula weight is not available
Natural source: Common Name: sperm whale Taxonomy ID: 9755 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Physeter catodon
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
myoglobin: VLSEGEWQLVLHVWAKVEAD
VAGHGQDILIRLFKSHPETL
EKFDRFKHLKTEAEMKASED
LKKHGVTVLTALGAILKKKG
HHEAELKPLAQSHATKHKIP
IKYLEFISEAIIHVLHSRHP
GDFGADAQGAMNKALELFRK
DIAAKYKELGYQG
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 286 |
13C chemical shifts | 429 |
15N chemical shifts | 149 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | apoMb | 1 |
Entities:
Entity 1, apoMb 153 residues - Formula weight is not available
1 | VAL | LEU | SER | GLU | GLY | GLU | TRP | GLN | LEU | VAL | ||||
2 | LEU | HIS | VAL | TRP | ALA | LYS | VAL | GLU | ALA | ASP | ||||
3 | VAL | ALA | GLY | HIS | GLY | GLN | ASP | ILE | LEU | ILE | ||||
4 | ARG | LEU | PHE | LYS | SER | HIS | PRO | GLU | THR | LEU | ||||
5 | GLU | LYS | PHE | ASP | ARG | PHE | LYS | HIS | LEU | LYS | ||||
6 | THR | GLU | ALA | GLU | MET | LYS | ALA | SER | GLU | ASP | ||||
7 | LEU | LYS | LYS | HIS | GLY | VAL | THR | VAL | LEU | THR | ||||
8 | ALA | LEU | GLY | ALA | ILE | LEU | LYS | LYS | LYS | GLY | ||||
9 | HIS | HIS | GLU | ALA | GLU | LEU | LYS | PRO | LEU | ALA | ||||
10 | GLN | SER | HIS | ALA | THR | LYS | HIS | LYS | ILE | PRO | ||||
11 | ILE | LYS | TYR | LEU | GLU | PHE | ILE | SER | GLU | ALA | ||||
12 | ILE | ILE | HIS | VAL | LEU | HIS | SER | ARG | HIS | PRO | ||||
13 | GLY | ASP | PHE | GLY | ALA | ASP | ALA | GLN | GLY | ALA | ||||
14 | MET | ASN | LYS | ALA | LEU | GLU | LEU | PHE | ARG | LYS | ||||
15 | ASP | ILE | ALA | ALA | LYS | TYR | LYS | GLU | LEU | GLY | ||||
16 | TYR | GLN | GLY |
Samples:
sample_1: myoglobin, [U-95% 13C; U-90% 15N; U-80% 2H], 0.3 mM; sodium acetate, [U-90% 2H], 10 mM; ethanol, [U-90% 2H], 10 % by volume
ex-cond_1: pH: 4.1; temperature: 323 K; ionic strength: 0.005 M
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
HNCO | sample_1 | not available | ex-cond_1 |
H(CA)CO(CA)NH | sample_1 | not available | ex-cond_1 |
HNCA | sample_1 | not available | ex-cond_1 |
HN(CO)CA | sample_1 | not available | ex-cond_1 |
HN(CA)CB | sample_1 | not available | ex-cond_1 |
CBCA(CO)NH | sample_1 | not available | ex-cond_1 |
1H-15N HSQC-NOESY-HSQC | sample_1 | not available | ex-cond_1 |
Software:
FELIX v4.2 - data processing
NMR spectrometers:
- Bruker DMX 750 MHz
Related Database Links:
BMRB | 1027 1029 1200 1413 1455 1457 1459 1461 1463 1465 1467 1469 1471 15589 16217 16218 16499 16500 16501 1752 2345 2346 2347 2348 2431 2432 2433 2434 291 292 293 40 4061 4062 426 4676 4695 |
PDB | |
DBJ | BAF03579 |
GB | AAA72199 |
PRF | 742482A |
REF | NP_001277651 |
SP | P02185 |
AlphaFold | P02185 |
Download HSQC peak lists in one of the following formats:
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or all simulated shifts