BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4575

Title: Letter to the Editor: Backbone resonance assignment of the N-terminal 24 kDa fragment of the gyrase B subunit from S. aureus complexed with novobiocin

Deposition date: 2000-01-26 Original release date: 2000-04-03

Authors: Klaus, Werner; Ross, Alfred; Gsell, Bernard; Senn, Hans

Citation: Klaus, Werner; Ross, Alfred; Gsell, Bernard; Senn, Hans. "Letter to the Editor: Backbone Resonance Assignment of the N-terminal 24 kDa Fragment of the Gyrase B Subunit from S. aureus Complexed with Novobiocin"  J. Biomol. NMR 16, 357-358 (2000).

Assembly members:
gyrase B fragment, polymer, 211 residues, 24000 Da.
NOVOBIOCIN, non-polymer, 612.624 Da.

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:   Production method: recombinant technology   Host organism: Escherichi coli

Entity Sequences (FASTA):
gyrase B fragment: GLEVVRKRPGMYIGSTSERG LHHLVWEIVDNSIDEALAGY ANQIEVVIEKDNWIKVTDNG RGIPVDIQEKMGRPAVEVIL TVLHAGGKFGGGGYKVSGGL HGVGSSVVNALSQDLEVYVH RNETIYHQAYKKGVPQFDLK EVGTTDKTGTVIRFKADGEI FTETTVYNYETLQQRIRELA FLNKGIQITLRDERDEENVR EDSYHYEGGIK

Data sets:
Data typeCount
13C chemical shifts583
15N chemical shifts202
1H chemical shifts582

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1gyrB1
2novobiocin2

Entities:

Entity 1, gyrB 211 residues - 24000 Da.

1   GLYLEUGLUVALVALARGLYSARGPROGLY
2   METTYRILEGLYSERTHRSERGLUARGGLY
3   LEUHISHISLEUVALTRPGLUILEVALASP
4   ASNSERILEASPGLUALALEUALAGLYTYR
5   ALAASNGLNILEGLUVALVALILEGLULYS
6   ASPASNTRPILELYSVALTHRASPASNGLY
7   ARGGLYILEPROVALASPILEGLNGLULYS
8   METGLYARGPROALAVALGLUVALILELEU
9   THRVALLEUHISALAGLYGLYLYSPHEGLY
10   GLYGLYGLYTYRLYSVALSERGLYGLYLEU
11   HISGLYVALGLYSERSERVALVALASNALA
12   LEUSERGLNASPLEUGLUVALTYRVALHIS
13   ARGASNGLUTHRILETYRHISGLNALATYR
14   LYSLYSGLYVALPROGLNPHEASPLEULYS
15   GLUVALGLYTHRTHRASPLYSTHRGLYTHR
16   VALILEARGPHELYSALAASPGLYGLUILE
17   PHETHRGLUTHRTHRVALTYRASNTYRGLU
18   THRLEUGLNGLNARGILEARGGLULEUALA
19   PHELEUASNLYSGLYILEGLNILETHRLEU
20   ARGASPGLUARGASPGLUGLUASNVALARG
21   GLUASPSERTYRHISTYRGLUGLYGLYILE
22   LYS

Entity 2, novobiocin - C31 H36 N2 O11 - 612.624 Da.

1   NOV

Samples:

sample_1: gyrase B fragment, [U-13C; U-15N], 1.2 mM

gyrB: pH: 6.5; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availablegyrB
HNCOsample_1not availablegyrB
CBCA(CO)NHsample_1not availablegyrB
HNCACBsample_1not availablegyrB
HBHA(CO)NHsample_1not availablegyrB
15N-edited NOESY-HSQCsample_1not availablegyrB

Software:

nmrPipe -

GARANT -

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

PDB
DBJ BAA01369 BAB41221 BAB56167 BAB93870 BAC11788
EMBL CAA50570 CAG39033 CAG41777 CAI79693 CAQ48445
GB AAW37393 ABD21942 ABD29196 ABD52436 ABQ47819
REF WP_000255576 WP_000255577 WP_000255578 WP_000255579 WP_000255580
SP P0A0K7 P0A0K8 P66936 P66937 Q2FKQ1
AlphaFold P66937 Q2FKQ1 P0A0K8 P0A0K7 P66936

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts