BMRB Entry 4633
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Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4633
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Title: Solution Structure, Backbone Dynamics, and stability of a Double Mutant Single-Chain Monellin. Structural origin of sweetness PubMed: 11279156
Deposition date: 2000-09-18 Original release date: 2002-12-23
Authors: Sung, Y.; Shin, J.; Chang, H.; Cho, J.; Lee, W.
Citation: Sung, Y.; Shin, J.; Chang, H.; Cho, J.; Lee, W.. "Solution Structure, Backbone Dynamics, and stability of a Double Mutant Single-Chain Monellin. Structural origin of sweetness " J. Biol. Chem. 276, 19624-19630 (2001).
Assembly members:
MONELLIN, polymer, 91 residues, Formula weight is not available
Natural source: Common Name: Serendipity berry Taxonomy ID: 3457 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Dioscoreophyllum cumminsii
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET21
Entity Sequences (FASTA):
MONELLIN: GEWEIIEIGPFYQNLGKFAV
DEENKIGQYGRLTFNKVIKP
CMKKTIYENEREIKGYEYQL
YVYASDKLFRADISEDYKTR
GRKLLRFNGPV
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 596 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | MONELLIN | 1 |
Entities:
Entity 1, MONELLIN 91 residues - Formula weight is not available
| 1 | GLY | GLU | TRP | GLU | ILE | ILE | GLU | ILE | GLY | PRO | ||||
| 2 | PHE | TYR | GLN | ASN | LEU | GLY | LYS | PHE | ALA | VAL | ||||
| 3 | ASP | GLU | GLU | ASN | LYS | ILE | GLY | GLN | TYR | GLY | ||||
| 4 | ARG | LEU | THR | PHE | ASN | LYS | VAL | ILE | LYS | PRO | ||||
| 5 | CYS | MET | LYS | LYS | THR | ILE | TYR | GLU | ASN | GLU | ||||
| 6 | ARG | GLU | ILE | LYS | GLY | TYR | GLU | TYR | GLN | LEU | ||||
| 7 | TYR | VAL | TYR | ALA | SER | ASP | LYS | LEU | PHE | ARG | ||||
| 8 | ALA | ASP | ILE | SER | GLU | ASP | TYR | LYS | THR | ARG | ||||
| 9 | GLY | ARG | LYS | LEU | LEU | ARG | PHE | ASN | GLY | PRO | ||||
| 10 | VAL |
Samples:
sample_1: MONELLIN 2 mM; photassium phosphate 50 mM; H20 90%; D20 10%
sample_2: MONELLIN, [U-15N], 2 mM; photassium phosphate 50 mM; H20 90%; D20 10%
sample_3: MONELLIN 2 mM; photassium phosphate 50 mM; D20 100%
sample_4: MONELLIN, [U-15N], 2 mM; photassium phosphate 50 mM; D20 100%
sample_cond_1: pH: 7.0; temperature: 298 K; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | sample_cond_1 |
| 3D 15N-separated NOESY | not available | not available | sample_cond_1 |
| DQF-COSY | not available | not available | sample_cond_1 |
| HNHA | not available | not available | sample_cond_1 |
| HSQC(exchange) | not available | not available | sample_cond_1 |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 500 MHz
Related Database Links:
| PDB |