BMRB Entry 4666
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4666
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Biotin carboxyl carrier domain of transcarboxylase (TC 1.3S)
Deposition date: 2000-02-08 Original release date: 2000-05-26
Authors: Reddy, D.; Shenoy, B.; Carey, P.; Sonnichsen, F.
Citation: Reddy, D.; Shenoy, B.; Carey, P.; Sonnichsen, F.. "High Resolution Solution Structure of the 1.3S Subunit of Transcarboxylase from Propionibacterium shermanii" Biochemistry 39, 2509-2516 (2000).
Assembly members:
TRANSCARBOXYLASE 1.3S SUBUNIT, polymer, 77 residues, 12367 Da.
NORBIOTIN, non-polymer, 230.284 Da.
Natural source: Common Name: Propionibacterium Freudenreichii subsp shermanii Taxonomy ID: 1752 Superkingdom: Bacteria Kingdom: not available Genus/species: Propionibacterium Freudenreichii subsp shermanii
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
TRANSCARBOXYLASE 1.3S SUBUNIT: AGAGKAGEGEIPAPLAGTVS
KILVKEGDTVKAGQTVLVLE
AMKMETEINAPTDGKVEKVL
VKERDAVQGGQGLIKIG
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 15N chemical shifts | 80 |
| 1H chemical shifts | 545 |
| 13C chemical shifts | 247 |
| coupling constants | 47 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | transcarboxylase 1.3S subunit | 1 |
| 2 | biotin moiety | 2 |
Entities:
Entity 1, transcarboxylase 1.3S subunit 77 residues - 12367 Da.
| 1 | ALA | GLY | ALA | GLY | LYS | ALA | GLY | GLU | GLY | GLU | ||||
| 2 | ILE | PRO | ALA | PRO | LEU | ALA | GLY | THR | VAL | SER | ||||
| 3 | LYS | ILE | LEU | VAL | LYS | GLU | GLY | ASP | THR | VAL | ||||
| 4 | LYS | ALA | GLY | GLN | THR | VAL | LEU | VAL | LEU | GLU | ||||
| 5 | ALA | MET | LYS | MET | GLU | THR | GLU | ILE | ASN | ALA | ||||
| 6 | PRO | THR | ASP | GLY | LYS | VAL | GLU | LYS | VAL | LEU | ||||
| 7 | VAL | LYS | GLU | ARG | ASP | ALA | VAL | GLN | GLY | GLY | ||||
| 8 | GLN | GLY | LEU | ILE | LYS | ILE | GLY |
Entity 2, biotin moiety - C9 H14 N2 O3 S - 230.284 Da.
| 1 | SNR |
Samples:
sample_1: TRANSCARBOXYLASE 1.3S SUBUNIT, [U-95%13C; U-90%15N], 1 – 2 mM
sample_2: TRANSCARBOXYLASE 1.3S SUBUNIT, [U-90% 15N], 1 – 2 mM
sample_cond_1: pH: 6.7; temperature: 293 K; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | not available | not available | sample_cond_1 |
Software:
NMRPIPE vSGI6X.M4 - processing
PIPP v3.7.3 - data analysis
X-PLOR v3.81 - structure solution
NMR spectrometers:
- Varian Inova 500 MHz
- Varian Inova 600 MHz
Related Database Links:
| BMRB | 4667 |
| PDB | |
| EMBL | CAD59402 CBL57376 CDP49157 CEG86413 CEG88429 |
| GB | AAA25674 AAA89090 AAA89092 AAA89094 AJQ91496 |
| REF | WP_013161729 WP_048769525 |
| SP | P02904 |
| AlphaFold | P02904 P02904 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts