BMRB Entry 4685
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4685
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Title: Backbone and side-chain 1H chemical shifts for J-atracotoxin-Hv1c
Deposition date: 2000-03-10 Original release date: 2000-03-23
Authors: Wang, Xiu-hong; King, Glenn
Citation: Wang, Xiu-hong; Smith, Ross; Connor, Mark; Maciejewski, Mark; Howden, Merlin; Nicholson, Graham; Christie, Macdonald; King, Glenn. "Discovery and characterization of a family of insecticidal neurotoxins with a rare vicinal disulfide bridge" .
Assembly members:
J-atracotoxin-Hv1c, polymer, 37 residues, 3761 Da.
Natural source: Common Name: Australian funnel-web spider Taxonomy ID: 6904 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Hadronyche versuta
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
J-atracotoxin-Hv1c: AICTGADRPCAACCPCCPGT
SCKAESNGVSYCRKDEP
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 209 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | J-ACTX-Hv1c | 1 |
Entities:
Entity 1, J-ACTX-Hv1c 37 residues - 3761 Da.
| 1 | ALA | ILE | CYS | THR | GLY | ALA | ASP | ARG | PRO | CYS | ||||
| 2 | ALA | ALA | CYS | CYS | PRO | CYS | CYS | PRO | GLY | THR | ||||
| 3 | SER | CYS | LYS | ALA | GLU | SER | ASN | GLY | VAL | SER | ||||
| 4 | TYR | CYS | ARG | LYS | ASP | GLU | PRO |
Samples:
sample_1: J-atracotoxin-Hv1c 1.6 mM
Ex-cond_1: pH: 4.95; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| TOCSY | sample_1 | not available | Ex-cond_1 |
| NOESY | sample_1 | not available | Ex-cond_1 |
| ECOSY | sample_1 | not available | Ex-cond_1 |
Software:
xwinnmr v2.0 - Spectral acquisition and processing
XEASY v1.3.13 - Resonance assignment
NMR spectrometers:
- Bruker Avance 600 MHz