BMRB Entry 4702
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4702
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Title: Structure of the Negative Regulatory Domain of p53 bound to S100B
Deposition date: 2000-03-27 Original release date: 2000-12-18
Authors: Rustandi, Richard; Baldisseri, Donna; Weber, David
Citation: Rustandi, Richard; Baldisseri, Donna; Weber, David. "Structure of the Negative Regulatory Domain of p53 bound to S100B" Nat. Struct. Biol. 7, 570-574 (2000).
Assembly members:
S100B beta, polymer, 91 residues, Formula weight is not available
C-terminal p53, polymer, 24 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.
Natural source: Common Name: rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus novergicus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
S100B beta: SELEKAMVALIDVFHQYSGR
EGDKHKLKKSELKELINNEL
SHFLEEIKEQEVVDKVMETL
DEDGDGECDFQEFMAFVSMV
TTACHEFFEHE
C-terminal p53: XSHLKSKKGQSTSRHKKLMF
KTEX
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 113 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | a chain | 1 |
| 2 | b chain | 1 |
| 3 | Ca 1 | 3 |
| 4 | Ca 2 | 3 |
| 5 | C-terminal p53 1 | 2 |
| 6 | C-terminal p53 2 | 2 |
Entities:
Entity 1, a chain 91 residues - Formula weight is not available
| 1 | SER | GLU | LEU | GLU | LYS | ALA | MET | VAL | ALA | LEU | ||||
| 2 | ILE | ASP | VAL | PHE | HIS | GLN | TYR | SER | GLY | ARG | ||||
| 3 | GLU | GLY | ASP | LYS | HIS | LYS | LEU | LYS | LYS | SER | ||||
| 4 | GLU | LEU | LYS | GLU | LEU | ILE | ASN | ASN | GLU | LEU | ||||
| 5 | SER | HIS | PHE | LEU | GLU | GLU | ILE | LYS | GLU | GLN | ||||
| 6 | GLU | VAL | VAL | ASP | LYS | VAL | MET | GLU | THR | LEU | ||||
| 7 | ASP | GLU | ASP | GLY | ASP | GLY | GLU | CYS | ASP | PHE | ||||
| 8 | GLN | GLU | PHE | MET | ALA | PHE | VAL | SER | MET | VAL | ||||
| 9 | THR | THR | ALA | CYS | HIS | GLU | PHE | PHE | GLU | HIS | ||||
| 10 | GLU |
Entity 3, Ca 1 - Ca - 40.078 Da.
| 1 | CA |
Entity 2, C-terminal p53 1 24 residues - Formula weight is not available
| 1 | ACE | SER | HIS | LEU | LYS | SER | LYS | LYS | GLY | GLN | ||||
| 2 | SER | THR | SER | ARG | HIS | LYS | LYS | LEU | MET | PHE | ||||
| 3 | LYS | THR | GLU | NH2 |
Samples:
sample_1: C-terminal p533 – 7 mM
condition_1: pH: 6.5; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 12C filter NOESY and TOCSY | sample_1 | not available | condition_1 |
| 2D 12C, 14N filter NOESY and TOCSY | sample_1 | not available | condition_1 |
| 2D NOESY and 2D TOCSY | sample_1 | not available | condition_1 |
| 2D 14N filter NOESY and TOCSY | sample_1 | not available | condition_1 |
Software:
NMRPipe v2.0 - Process and analyse the NMR data
NMR spectrometers:
- Bruker DMX 600 MHz
Related Database Links:
| BMRB | 15923 4001 4099 4105 4099 |
| PDB | |
| DBJ | BAB43945 BAE22214 BAE22413 BAE36647 BAE88979 BAC16799.1 BAD96746.1 |
| EMBL | CAA25567 CAG46920 CAA42635.1 CAA42627.1 CAA38095.1 CAA26306.1 CAJ28922.1 |
| GB | AAA03075 AAA42096 AAA60367 AAH01766 AAH61178 |
| PRF | 2003367B |
| REF | NP_001076199 NP_001233735 NP_001247455 NP_001270589 NP_006263 XP_511957.2 NP_000537.3 XP_001172077.1 XP_001172061.1 XP_001172050.1 |
| SP | P04271 P04631 P50114 Q6YNR6 |
| SWISS-PROT | P04637 |
| GenBank | EAW90142.1 EAW90140.1 ABM86630.1 ABL09846.1 EAW90141.1 |
| AlphaFold | P04631 P04271 P50114 Q6YNR6 |