BMRB Entry 4704

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4704

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Analysis of the dynamic properties of Bacillus circulans xylanase upon formation of a covalent glycosyl-enzyme intermediate

Deposition date:

2000-03-30

Original release date:

2000-05-08

Authors:

Connelly, Gregory; Withers, Stephen; McIntosh, Lawrence

Citation:

Citation: Connelly, Gregory; Withers, Stephen; McIntosh, Lawrence. "Analysis of the dynamic properties of Bacillus circulans xylanase upon formation of a covalent glycosyl-enzyme intermediate" Protein Sci. 9, 512-524 (2000).

Assembly members:

Assembly members:
xylanase, polymer, 185 residues, 20396 Da.

Natural source:

Natural source: Common Name: Bacillus circulans Taxonomy ID: 1397 Superkingdom: Eubacteria Kingdom: not available Genus/species: Bacillus circulans

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
xylanase: ASTDYWQNWTDGGGIVNAVN GSGGNYSVNWSNTGNFVVGK GWTTGSPFRTINYNAGVWAP NGNGYLTLYGWTRSPLIEYY VVDSWGTYRPTGTYKGTVKS DGGTYDIYTTTRYNAPSIDG DRTTFTQYWSVRQTKRPTGS NATITFSNHVNAWKSHGMNL GSNWAYQVMATEGYQSSGSS NVTVW

Data sets:

assigned_chemical_shifts
- chemical_shift_assignment_one

Data type	Count
1H chemical shifts	177
13C chemical shifts	341
15N chemical shifts	177

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	BCX	1

Entities:

Entity 1, BCX 185 residues - 20396 Da.

1

ALA

SER

THR

ASP

TYR

TRP

GLN

ASN

TRP

THR

2

ASP

GLY

ILE

VAL

ASN

ALA

VAL

ASN

3

GLY

SER

GLY

ASN

TYR

SER

VAL

ASN

TRP

4

SER

ASN

THR

GLY

ASN

PHE

VAL

GLY

LYS

5

GLY

TRP

THR

GLY

SER

PRO

PHE

ARG

THR

6

ILE

ASN

TYR

ASN

ALA

GLY

VAL

TRP

ALA

PRO

7

ASN

GLY

ASN

GLY

TYR

LEU

THR

LEU

TYR

GLY

8

TRP

THR

ARG

SER

PRO

LEU

ILE

GLU

TYR

9

VAL

ASP

SER

TRP

GLY

THR

TYR

ARG

PRO

10

THR

GLY

THR

TYR

LYS

GLY

THR

VAL

LYS

SER

11

ASP

GLY

THR

TYR

ASP

ILE

TYR

THR

12

THR

ARG

TYR

ASN

ALA

PRO

SER

ILE

ASP

GLY

13

ASP

ARG

THR

PHE

THR

GLN

TYR

TRP

SER

14

VAL

ARG

GLN

THR

LYS

ARG

PRO

THR

GLY

SER

15

ASN

ALA

THR

ILE

THR

PHE

SER

ASN

HIS

VAL

16

ASN

ALA

TRP

LYS

SER

HIS

GLY

MET

ASN

LEU

17

GLY

SER

ASN

TRP

ALA

TYR

GLN

VAL

MET

ALA

18

THR

GLU

GLY

TYR

GLN

SER

GLY

SER

19

ASN

VAL

THR

VAL

TRP

Samples:

apo-BCX: xylanase, [U-99% 13C; U-99% 15N], 0.9 mM

Conditions_1: pH: 5.7; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCACB	apo-BCX	not available	Conditions_1
CBCACONH	apo-BCX	not available	Conditions_1

Software:

Felix v95 - data processing peak assignments

NMR spectrometers:

Varian Unity 500 MHz

BMRB	4705
PDB	1AXK 1BCX 1BVV 1C5H 1C5I 1HV0 1HV1 1XNB 1XNC 1XXN 2B42 2B45 2B46 2BVV 2DCY 2DCZ 2QZ3 2Z79 3EXU 3HD8 3VZJ 3VZK 3VZL 3VZM 3VZN 3VZO
DBJ	BAH28803 BAM52542 BAM58117
EMBL	CAA30553 CAA41783 CAA84276 CAB13776 CCU58510
GB	AAA22897 AAB84458 AAM08359 AAM08360 AAW83159
PRF	1209158A
REF	NP_389765 WP_003231377 WP_014477026 WP_014664226 WP_015251938
SP	P09850 P18429
AlphaFold	P09850 P18429