BMRB Entry 4710
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4710
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Citation: Laity, John; Chung, John; Dyson, H.; Wright, Peter. "Alternative Splicing of Wilms' Tumor Suppressor Protein Modulates DNA Binding
Activity Through Isoform-Specific DNA-Induced Conformational Changes" Biochemistry ., .-. (2000).
Assembly members:
WT1, polymer, 122 residues, 14766 Da.
DNA, polymer, 14 residues, Formula weight is not available
DNA strand 2, polymer, 14 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: not available Genus/species: Homo Sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET21
Entity Sequences (FASTA):
WT1: ASEKRPFMCAYPGCNKRYFK
LSHLQMHSRKHTGEKPYQCD
FKDCERRFSRSDQLKRHQRR
HTGVKPFQCKTCQRKFSRSD
HLKTHTRTHTGKTSEKPFSC
RWPSCQKKFARSDELVRHHN
MH
DNA: CGCGGGGGCGTCTG
DNA strand 2: CAGACGCCCCCGCG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 222 |
| 15N chemical shifts | 97 |
| 1H chemical shifts | 97 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | WT1+KTS | 1 |
| 2 | DNA strand 1 | 2 |
| 3 | DNA strand 2 | 3 |
| 4 | Zn 1 | 4 |
| 5 | Zn 2 | 4 |
| 6 | Zn 3 | 4 |
| 7 | Zn 4 | 4 |
Entities:
Entity 1, WT1+KTS 122 residues - 14766 Da.
| 1 | ALA | SER | GLU | LYS | ARG | PRO | PHE | MET | CYS | ALA | ||||
| 2 | TYR | PRO | GLY | CYS | ASN | LYS | ARG | TYR | PHE | LYS | ||||
| 3 | LEU | SER | HIS | LEU | GLN | MET | HIS | SER | ARG | LYS | ||||
| 4 | HIS | THR | GLY | GLU | LYS | PRO | TYR | GLN | CYS | ASP | ||||
| 5 | PHE | LYS | ASP | CYS | GLU | ARG | ARG | PHE | SER | ARG | ||||
| 6 | SER | ASP | GLN | LEU | LYS | ARG | HIS | GLN | ARG | ARG | ||||
| 7 | HIS | THR | GLY | VAL | LYS | PRO | PHE | GLN | CYS | LYS | ||||
| 8 | THR | CYS | GLN | ARG | LYS | PHE | SER | ARG | SER | ASP | ||||
| 9 | HIS | LEU | LYS | THR | HIS | THR | ARG | THR | HIS | THR | ||||
| 10 | GLY | LYS | THR | SER | GLU | LYS | PRO | PHE | SER | CYS | ||||
| 11 | ARG | TRP | PRO | SER | CYS | GLN | LYS | LYS | PHE | ALA | ||||
| 12 | ARG | SER | ASP | GLU | LEU | VAL | ARG | HIS | HIS | ASN | ||||
| 13 | MET | HIS |
Entity 2, DNA strand 1 14 residues - Formula weight is not available
| 1 | DC | DG | DC | DG | DG | DG | DG | DG | DC | DG | ||||
| 2 | DT | DC | DT | DG |
Entity 3, DNA strand 2 14 residues - Formula weight is not available
| 1 | DC | DA | DG | DA | DC | DG | DC | DC | DC | DC | ||||
| 2 | DC | DG | DC | DG |
Entity 4, Zn 1 - Zn - 65.409 Da.
| 1 | ZN |
Related Database Links:
| BMRB | 15532 15533 4707 4708 4709 |
| PDB | 2JP9 2JPA 2PRT |
| DBJ | BAA28147 BAA76399 BAA94794 BAF84425 BAG52667 |
| EMBL | CAA35956 CAA49373 CAA59735 CAA59737 CDG23662 |
| GB | AAA40573 AAA61299 AAB27319 AAB33427 AAB70832 |
| PRF | 1604420A |
| REF | NP_000369 NP_001001264 NP_001185481 NP_077742 NP_077744 |
| SP | B7ZSG3 O62651 P19544 P22561 P49952 |
| TPG | DAA21902 |
| AlphaFold | B7ZSG3 O62651 P19544 P22561 P49952 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks