BMRB Entry 4711
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4711
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: NMR backbone assignments of the cold-regulated RNA-binding protein, RbpA1, in the cyanobacterium, Anabaena variabilis M3
Deposition date: 2000-04-10 Original release date: 2000-09-25
Authors: Morita, Hayato; Murakami, Toshihide; Uegaki, Koichi; Yamazaki, Toshio; Sato, Naoki; Kyogoku, Yoshimasa; Hayashi, Hidenori
Citation: Morita, Eugene; Murakami, Toshihide; Uegaki, Koichi; Yamazaki, Toshio; Sato, Naoki; Kyogoku, Yoshimasa; Hayashi, Hidenori. "Letter to the Editor: NMR backbone assignments of the cold-regulated RNA-binding protein, RbpA1, in the cyanobacterium, Anabaena variabilis M3" J. Biomol. NMR 17, 351-352 (2000).
Assembly members:
RNA-binding protein, polymer, 101 residues, Formula weight is not available
Natural source: Common Name: Anabaena variabilis M3 Taxonomy ID: 1172 Superkingdom: Eubacteria Kingdom: not available Genus/species: Anabaena variabilis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: peT-21b
Entity Sequences (FASTA):
RNA-binding protein: SIYVGNLSYEVTQDTLSAVF
AEYGTVKRVQLPTDRETGQP
RGFGFVEMGSEAEEAAAIEA
LDGAEWMGRDLKVNKAKPRE
DRGPSGGNRGGYGGGGGRNR
Y
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 101 |
| 15N chemical shifts | 96 |
| 1H chemical shifts | 96 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RNA-binding protein | 1 |
Entities:
Entity 1, RNA-binding protein 101 residues - Formula weight is not available
| 1 | SER | ILE | TYR | VAL | GLY | ASN | LEU | SER | TYR | GLU | ||||
| 2 | VAL | THR | GLN | ASP | THR | LEU | SER | ALA | VAL | PHE | ||||
| 3 | ALA | GLU | TYR | GLY | THR | VAL | LYS | ARG | VAL | GLN | ||||
| 4 | LEU | PRO | THR | ASP | ARG | GLU | THR | GLY | GLN | PRO | ||||
| 5 | ARG | GLY | PHE | GLY | PHE | VAL | GLU | MET | GLY | SER | ||||
| 6 | GLU | ALA | GLU | GLU | ALA | ALA | ALA | ILE | GLU | ALA | ||||
| 7 | LEU | ASP | GLY | ALA | GLU | TRP | MET | GLY | ARG | ASP | ||||
| 8 | LEU | LYS | VAL | ASN | LYS | ALA | LYS | PRO | ARG | GLU | ||||
| 9 | ASP | ARG | GLY | PRO | SER | GLY | GLY | ASN | ARG | GLY | ||||
| 10 | GLY | TYR | GLY | GLY | GLY | GLY | GLY | ARG | ASN | ARG | ||||
| 11 | TYR |
Samples:
sample_1: RNA-binding protein, [U-13C; U-15N], 0.5 mM; H2O 90%; D2O 10%
cond_1: pH: 6.9; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N_HSQC | not available | not available | not available |
| 3D HNCACB | not available | not available | not available |
| 3D CBCA(CO)NH | not available | not available | not available |
Software:
NMRPipe v1.6, NIH - peak picking and assignments
NMR spectrometers:
- Bruker DMX 500 MHz
Related Database Links:
| DBJ | BAA04563 BAB72698 |
| GB | ABA24239 |
| REF | WP_010994915 |
| SP | Q44560 |
| AlphaFold | Q44560 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts