BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4758

Title: A novel killer toxin-like protein, SKLP, is a member of the single-domain beta-gamma crystallin family proteins   PubMed: 11114251

Deposition date: 2000-06-12 Original release date: 2001-05-01

Authors: Ohki, Shin-ya; Kariya, Eri; Hiraga, Kazumi; Wakamiya, Akiko; Isobe, Toshiaki; Oda, Kohei; Kainosho, Masatsune

Citation: Ohki, Shin-ya; Kariya, Eri; Hiraga, Kazumi; Wakamiya, Akiko; Isobe, Toshiaki; Oda, Kohei; Kainosho, Masatsune. "NMR Structure of Streptomyces Killer Toxin-like Protein, SKLP: Further Evidence for the Wide Distribution of Single-domain Betagamma-crystallin Superfamily Proteins"  J. Mol. Biol. 305, 109-120 (2001).

Assembly members:
yeast killer toxin-like protein, polymer, 84 residues, Formula weight is not available

Natural source:   Common Name: Streptomyces sp. F-287   Taxonomy ID: 1883   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Streptomyces sp. F-287

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
yeast killer toxin-like protein: IDHVPCRGGENFLKIWSHSG GQQSVDCYANRGRIDFGGWW VDKISTGNNDLIYYDANGDS VRVDRWHDITYPNRPPKVNS IEIL

Data sets:
Data typeCount
13C chemical shifts254
15N chemical shifts86
1H chemical shifts436

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SKLP monomer1

Entities:

Entity 1, SKLP monomer 84 residues - Formula weight is not available

1   ILEASPHISVALPROCYSARGGLYGLYGLU
2   ASNPHELEULYSILETRPSERHISSERGLY
3   GLYGLNGLNSERVALASPCYSTYRALAASN
4   ARGGLYARGILEASPPHEGLYGLYTRPTRP
5   VALASPLYSILESERTHRGLYASNASNASP
6   LEUILETYRTYRASPALAASNGLYASPSER
7   VALARGVALASPARGTRPHISASPILETHR
8   TYRPROASNARGPROPROLYSVALASNSER
9   ILEGLUILELEU

Samples:

sample_1: yeast killer toxin-like protein 1.0 mM

conditions_1: pH: 6.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

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