BMRB Entry 4768
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4768
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Title: Structure of parvulin hPar14
Deposition date: 2000-06-22 Original release date: 2000-12-07
Authors: Sekerina, Elena; Rahfeld, Jens; Muller, Jonathan; Fanghanel, Jorg; Rascher, Christine; Fischer, Gunter; Bayer, Peter
Citation: Sekerina, Elena; Rahfeld, Jens; Muller, Jonathan; Fanghanel, Jorg; Rascher, Christine; Fischer, Gunter; Bayer, Peter. "NMR Solution Structure of hPar14 Reveals Similarity to the Peptidyl Prolyl cis/tans Isomerase Domain of the Mitotic Regulator hPin1 but Indicates a Different Functionality of the Protein" J. Mol. Biol. 301, 1003-1017 (2000).
Assembly members:
human parvulin 14, polymer, 131 residues, 13809.9 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
human parvulin 14: MPPKGKSGSGKAGKGGAASG
SDSADKKAQGPKGGGNAVKV
RHILCEKHGKIMEAMEKLKS
GMRFNEVAAQYSEDKARQGG
DLGWMTRGSMVGPFQEAAFA
LPVSGMDKPVFTDPPVKTKF
GYHIIMVEGRK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 365 |
| 13C chemical shifts | 179 |
| 15N chemical shifts | 92 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hpar14 | 1 |
Entities:
Entity 1, hpar14 131 residues - 13809.9 Da.
| 1 | MET | PRO | PRO | LYS | GLY | LYS | SER | GLY | SER | GLY | ||||
| 2 | LYS | ALA | GLY | LYS | GLY | GLY | ALA | ALA | SER | GLY | ||||
| 3 | SER | ASP | SER | ALA | ASP | LYS | LYS | ALA | GLN | GLY | ||||
| 4 | PRO | LYS | GLY | GLY | GLY | ASN | ALA | VAL | LYS | VAL | ||||
| 5 | ARG | HIS | ILE | LEU | CYS | GLU | LYS | HIS | GLY | LYS | ||||
| 6 | ILE | MET | GLU | ALA | MET | GLU | LYS | LEU | LYS | SER | ||||
| 7 | GLY | MET | ARG | PHE | ASN | GLU | VAL | ALA | ALA | GLN | ||||
| 8 | TYR | SER | GLU | ASP | LYS | ALA | ARG | GLN | GLY | GLY | ||||
| 9 | ASP | LEU | GLY | TRP | MET | THR | ARG | GLY | SER | MET | ||||
| 10 | VAL | GLY | PRO | PHE | GLN | GLU | ALA | ALA | PHE | ALA | ||||
| 11 | LEU | PRO | VAL | SER | GLY | MET | ASP | LYS | PRO | VAL | ||||
| 12 | PHE | THR | ASP | PRO | PRO | VAL | LYS | THR | LYS | PHE | ||||
| 13 | GLY | TYR | HIS | ILE | ILE | MET | VAL | GLU | GLY | ARG | ||||
| 14 | LYS |
Samples:
sample1: human parvulin 141.0 – 2.0 mM
sample2: human parvulin 14, [U-13C; U-15N], 1.0 – 2.0 mM
cond-1: pH: 6.5; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
NDee - assignment of 2D spectra
Aurelia - assignment of 3D spectra
NMR spectrometers:
- Bruker DRX 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAA82320 BAB26863 BAG54532 |
| GB | AAD27893 AAH05234 AAH70288 AAH93700 AAI04654 |
| REF | NP_001164218 NP_006214 NP_081457 XP_001092957 XP_001119408 |
| SP | Q9CWW6 Q9Y237 |
| AlphaFold | Q9CWW6 Q9Y237 |
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