BMRB Entry 4784
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4784
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Title: 1H and 15N chemical shift assignments and interproton 3JHNHA coupling constants of the DNA-binding domain of the tyrosine repressor from Haemophilus influenzae, a transcription factor that belongs to the prokaryotic NtrC superfamily
Deposition date: 2000-07-13 Original release date: 2001-07-17
Authors: Wang, Yunjun; Zhao, Shimin; Somerville, Ronald; Jardetzky, Oleg
Citation: Wang, Yunjun; Zhao, Shimin; Somerville, Ronald; Jardetzky, Oleg. "Solution Structure of the DNA-binding Domain of the TyrR Protein of Haemophilus influenzae" Protein Sci. 10, 592-598 (2001).
Assembly members:
repressor, polymer, 61 residues, Formula weight is not available
Natural source: Common Name: Haemophilus influenzae Taxonomy ID: 727 Superkingdom: Eubacteria Kingdom: not available Genus/species: Haemophilus influenzae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pZZ257
Entity Sequences (FASTA):
repressor: SAVISLDEFENKTLDEIIGF
YEAQVLKLFYAEYPSTRKLA
QRLGVSHTAIANKLKQYGIG
K
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 407 |
| 15N chemical shifts | 63 |
| coupling constants | 34 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Tyr repressor DNA binding domain | 1 |
Entities:
Entity 1, Tyr repressor DNA binding domain 61 residues - Formula weight is not available
| 1 | SER | ALA | VAL | ILE | SER | LEU | ASP | GLU | PHE | GLU | ||||
| 2 | ASN | LYS | THR | LEU | ASP | GLU | ILE | ILE | GLY | PHE | ||||
| 3 | TYR | GLU | ALA | GLN | VAL | LEU | LYS | LEU | PHE | TYR | ||||
| 4 | ALA | GLU | TYR | PRO | SER | THR | ARG | LYS | LEU | ALA | ||||
| 5 | GLN | ARG | LEU | GLY | VAL | SER | HIS | THR | ALA | ILE | ||||
| 6 | ALA | ASN | LYS | LEU | LYS | GLN | TYR | GLY | ILE | GLY | ||||
| 7 | LYS |
Samples:
sample_1: repressor, [U-15N], 2.0 mM
conditions_1: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- Varian Unity plus 800 MHz
Related Database Links:
| PDB | |
| EMBL | CBW28709 CBY81959 CBY86469 |
| GB | AAC22069 ADO80156 ADO95632 AIB45125 EDJ91589 |
| REF | NP_438572 WP_005630049 WP_005634169 WP_005652170 WP_005656417 |
| SP | P44694 |
| AlphaFold | P44694 |
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