BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4789

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for TAZ2 domain of CBP

Deposition date: 2000-07-19 Original release date: 2000-10-23

Authors: De Guzman, Roberto; Liu, Hong; Martinez-Yamout, Maria; Dyson, H.; Wright, Peter

Citation: De Guzman, Roberto; Liu, Hong; Martinez-Yamout, Maria; Dyson, H.; Wright, Peter. "Solution Structure of the TAZ2(CH3) Domain of the Transcriptional Adaptor Protein CBP"  J. Mol. Biol. 303, 243-253 (2000).

Assembly members:
CBP TAZ2 domain, polymer, 87 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-3a

Entity Sequences (FASTA):
CBP TAZ2 domain: SPQESRRLSIQRCIQSLVHA CQCRNANCSLPSCQKMKRVV QHTKGCKRKTNGGCPVCKQL IALCCYHAKHCQENKCPVPF CLNIKHK

Data sets:
Data typeCount
1H chemical shifts514
13C chemical shifts354
15N chemical shifts103

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TAZ21
2ZN_2+2

Entities:

Entity 1, TAZ2 87 residues - Formula weight is not available

1   SERPROGLNGLUSERARGARGLEUSERILE
2   GLNARGCYSILEGLNSERLEUVALHISALA
3   CYSGLNCYSARGASNALAASNCYSSERLEU
4   PROSERCYSGLNLYSMETLYSARGVALVAL
5   GLNHISTHRLYSGLYCYSLYSARGLYSTHR
6   ASNGLYGLYCYSPROVALCYSLYSGLNLEU
7   ILEALALEUCYSCYSTYRHISALALYSHIS
8   CYSGLNGLUASNLYSCYSPROVALPROPHE
9   CYSLEUASNILELYSHISLYS

Entity 2, ZN_2+ - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: CBP TAZ2 domain, [U-15N], 1 – 1.5 mM; ZINC (II) ION mM

sample_2: CBP TAZ2 domain, [U-15N; U-13C], 1 – 1.5 mM; ZINC (II) ION mM

Ex-cond_1: pH: 5.8; temperature: 298 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
15N NOESYnot availablenot availableEx-cond_1
15N TOCSYnot availablenot availableEx-cond_1
13C NOESYnot availablenot availableEx-cond_1
HNCAnot availablenot availableEx-cond_1
HNCACBnot availablenot availableEx-cond_1
CBCACONHnot availablenot availableEx-cond_1
HCCONHnot availablenot availableEx-cond_1
CCONHnot availablenot availableEx-cond_1
HNHAnot availablenot availableEx-cond_1
HNHBnot availablenot availableEx-cond_1
HACAHB-COSYnot availablenot availableEx-cond_1
HNCOnot availablenot availableEx-cond_1

Software:

NMRVIEW v4.1 - data analysis

NMRpipe - data processing

NMRDraw - data processing

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker DMX 600 MHz
  • Bruker DRX 750 MHz

Related Database Links:

SWISS-PROT P45481 Q92793
REF NP_001088637 NP_004371 NP_001073315 NP_001082924 NP_001020603
PRF 1923401A
GenBank AAC51770 AAH86282 AAC17736 AAC51331 AAB28651
EMBL CAN87737 CAK11048 CAM56592 CAF96470 CAG06082
PDB
DBJ BAE06125

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts