BMRB Entry 4789
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4789
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for TAZ2 domain of CBP
Deposition date: 2000-07-19 Original release date: 2000-10-23
Authors: De Guzman, Roberto; Liu, Hong; Martinez-Yamout, Maria; Dyson, H.; Wright, Peter
Citation: De Guzman, Roberto; Liu, Hong; Martinez-Yamout, Maria; Dyson, H.; Wright, Peter. "Solution Structure of the TAZ2(CH3) Domain of the Transcriptional Adaptor Protein CBP" J. Mol. Biol. 303, 243-253 (2000).
Assembly members:
CBP TAZ2 domain, polymer, 87 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-3a
Entity Sequences (FASTA):
CBP TAZ2 domain: SPQESRRLSIQRCIQSLVHA
CQCRNANCSLPSCQKMKRVV
QHTKGCKRKTNGGCPVCKQL
IALCCYHAKHCQENKCPVPF
CLNIKHK
- assigned_chemical_shifts
Data type | Count |
1H chemical shifts | 514 |
13C chemical shifts | 354 |
15N chemical shifts | 103 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | TAZ2 | 1 |
2 | ZN_2+ | 2 |
Entities:
Entity 1, TAZ2 87 residues - Formula weight is not available
1 | SER | PRO | GLN | GLU | SER | ARG | ARG | LEU | SER | ILE | ||||
2 | GLN | ARG | CYS | ILE | GLN | SER | LEU | VAL | HIS | ALA | ||||
3 | CYS | GLN | CYS | ARG | ASN | ALA | ASN | CYS | SER | LEU | ||||
4 | PRO | SER | CYS | GLN | LYS | MET | LYS | ARG | VAL | VAL | ||||
5 | GLN | HIS | THR | LYS | GLY | CYS | LYS | ARG | LYS | THR | ||||
6 | ASN | GLY | GLY | CYS | PRO | VAL | CYS | LYS | GLN | LEU | ||||
7 | ILE | ALA | LEU | CYS | CYS | TYR | HIS | ALA | LYS | HIS | ||||
8 | CYS | GLN | GLU | ASN | LYS | CYS | PRO | VAL | PRO | PHE | ||||
9 | CYS | LEU | ASN | ILE | LYS | HIS | LYS |
Entity 2, ZN_2+ - Zn - 65.409 Da.
1 | ZN |
Samples:
sample_1: CBP TAZ2 domain, [U-15N], 1 1.5 mM; ZINC (II) ION mM
sample_2: CBP TAZ2 domain, [U-15N; U-13C], 1 1.5 mM; ZINC (II) ION mM
Ex-cond_1: pH: 5.8; temperature: 298 K; pressure: 1 atm
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
15N NOESY | not available | not available | Ex-cond_1 |
15N TOCSY | not available | not available | Ex-cond_1 |
13C NOESY | not available | not available | Ex-cond_1 |
HNCA | not available | not available | Ex-cond_1 |
HNCACB | not available | not available | Ex-cond_1 |
CBCACONH | not available | not available | Ex-cond_1 |
HCCONH | not available | not available | Ex-cond_1 |
CCONH | not available | not available | Ex-cond_1 |
HNHA | not available | not available | Ex-cond_1 |
HNHB | not available | not available | Ex-cond_1 |
HACAHB-COSY | not available | not available | Ex-cond_1 |
HNCO | not available | not available | Ex-cond_1 |
Software:
NMRVIEW v4.1 - data analysis
NMRpipe - data processing
NMRDraw - data processing
NMR spectrometers:
- Bruker AMX 500 MHz
- Bruker DMX 600 MHz
- Bruker DRX 750 MHz
Related Database Links:
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts