BMRB Entry 4795
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4795
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Title: Chemical Shift Assignments for Human D187N Gelsolin Domain 2
Deposition date: 2000-07-25 Original release date: 2000-11-13
Authors: Kazmirski, Steven; Howard, Mark; Isaacson, Rivka; Fersht, Alan
Citation: Kazmirski, Steven; Howard, Mark; Isaacson, Rivka; Fersht, Alan. "Elucidating the Mechanism of Familial Amyloidosis--Finnish Type: NMR Studies of Human Gelsolin Domain 2" Proc. Natl. Acad. Sci. U.S.A. 97, 10706-10711 (2000).
Assembly members:
Gelsolin Domain 2, polymer, 116 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapien
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Gelsolin Domain 2: HVVPNEVVVQRLFQVKGRRV
VRATEVPVSWESFNNGNCFI
LDLGNNIHQWCGSNSNRYER
LKATQVSKGIRDNERSGRAR
VHVSEEGTEPEAMLQVLGPK
PALPAGTEDTAKEDAA
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 342 |
| 13C chemical shifts | 201 |
| 15N chemical shifts | 94 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | D187N gelsolin domain 2 | 1 |
Entities:
Entity 1, D187N gelsolin domain 2 116 residues - Formula weight is not available
| 1 | HIS | VAL | VAL | PRO | ASN | GLU | VAL | VAL | VAL | GLN | ||||
| 2 | ARG | LEU | PHE | GLN | VAL | LYS | GLY | ARG | ARG | VAL | ||||
| 3 | VAL | ARG | ALA | THR | GLU | VAL | PRO | VAL | SER | TRP | ||||
| 4 | GLU | SER | PHE | ASN | ASN | GLY | ASN | CYS | PHE | ILE | ||||
| 5 | LEU | ASP | LEU | GLY | ASN | ASN | ILE | HIS | GLN | TRP | ||||
| 6 | CYS | GLY | SER | ASN | SER | ASN | ARG | TYR | GLU | ARG | ||||
| 7 | LEU | LYS | ALA | THR | GLN | VAL | SER | LYS | GLY | ILE | ||||
| 8 | ARG | ASP | ASN | GLU | ARG | SER | GLY | ARG | ALA | ARG | ||||
| 9 | VAL | HIS | VAL | SER | GLU | GLU | GLY | THR | GLU | PRO | ||||
| 10 | GLU | ALA | MET | LEU | GLN | VAL | LEU | GLY | PRO | LYS | ||||
| 11 | PRO | ALA | LEU | PRO | ALA | GLY | THR | GLU | ASP | THR | ||||
| 12 | ALA | LYS | GLU | ASP | ALA | ALA |
Samples:
sample_1: Gelsolin Domain 2, [U-13C; U-15N], 0.5 mM
Cond_1: pH: 7.2; temperature: 298 K; ionic strength: 20 mM
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 1H-15N TOCSY | not available | not available | not available |
| 1H-15N NOESY | not available | not available | not available |
| HNCACB | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker AMX 500 MHz
- Bruker DRX 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts