BMRB Entry 4863
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4863
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Title: SOLUTION STRUCTURE OF NUCLEOLIN RBD2 PubMed: 11023788
Deposition date: 2000-10-17 Original release date: 2001-11-14
Authors: Allain, F.; Gilbert, D.; Bouvet, P.; Feigon, J.
Citation: Allain, F.; Gilbert, D.; Bouvet, P.; Feigon, J.. "Solution Strcuture of the two N-terminal RNA-binding Domains of Nucleolin and NMR Study of the Interaction with its RNA Target" J. Mol. Biol. 303, 227-241 (2000).
Assembly members:
NUCLEOLIN RBD2, polymer, 96 residues, Formula weight is not available
Natural source: Common Name: golden hamster Taxonomy ID: 10036 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mesocricetus auratus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: BL21(DE3)
Entity Sequences (FASTA):
NUCLEOLIN RBD2: SHMLEDPCTSKKVRAARTLL
AKNLSFNITEDELKEVFEDA
LEIRLVSQDGKSKGIAYIEF
KSEADAEKNLEEKQGAEIDG
RSVSLYYTGEKGGTRG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 252 |
| 15N chemical shifts | 95 |
| 1H chemical shifts | 589 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NUCLEOLIN RBD2 | 1 |
Entities:
Entity 1, NUCLEOLIN RBD2 96 residues - Formula weight is not available
| 1 | SER | HIS | MET | LEU | GLU | ASP | PRO | CYS | THR | SER | ||||
| 2 | LYS | LYS | VAL | ARG | ALA | ALA | ARG | THR | LEU | LEU | ||||
| 3 | ALA | LYS | ASN | LEU | SER | PHE | ASN | ILE | THR | GLU | ||||
| 4 | ASP | GLU | LEU | LYS | GLU | VAL | PHE | GLU | ASP | ALA | ||||
| 5 | LEU | GLU | ILE | ARG | LEU | VAL | SER | GLN | ASP | GLY | ||||
| 6 | LYS | SER | LYS | GLY | ILE | ALA | TYR | ILE | GLU | PHE | ||||
| 7 | LYS | SER | GLU | ALA | ASP | ALA | GLU | LYS | ASN | LEU | ||||
| 8 | GLU | GLU | LYS | GLN | GLY | ALA | GLU | ILE | ASP | GLY | ||||
| 9 | ARG | SER | VAL | SER | LEU | TYR | TYR | THR | GLY | GLU | ||||
| 10 | LYS | GLY | GLY | THR | ARG | GLY |
Samples:
sample_1: NUCLEOLIN RBD2, [U-15N; U-13C], 1 mM; potassium phosphate 50 mM; KCl 100 mM; DTT, [U-2H], 5 mM; H2O 90%; D2O 10%
sample_2: NUCLEOLIN RBD2, [U-15N; U-13C], 1 mM; potassium phosphate 50 mM; KCl 100 mM; DTT, [U-2H], 5 mM; D2O 100%
sample_cond_1: ionic strength: 150 mM; pH: 6.2; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 13C-separated NOESY | not available | not available | not available |
| 3D 15N-separated NOESY | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
Software:
X-PLOR v3.841 - refinement, structure solution
DYANA v1 - structure solution
NMR spectrometers:
- Bruker DRX 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts