BMRB Entry 4870
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4870
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Title: Sequence-specific 1H, 15N, and 13C resonance assignments for the whole region 4 of Escherichia coli RNA polymerase sigma70 subunit
Deposition date: 2000-10-20 Original release date: 2011-08-10
Authors: Poznanski, Jaroslaw; Zhukow, Igor; Bolewska, Krystyna; Wierzchowski, Kazimierz
Citation: Poznanski, Jaroslaw; Zhukov, Igor; Bolewska, Krystyna; Wierzchowski, Kazimierz. "Letter to the Editor: Sequence-specific 1H, 15N, and 13C resonance assignments for the whole region 4 of Escherichia coli RNA polymerase sigma70 subunit" J. Biomol. NMR 20, 181-182 (2001).
Assembly members:
region 4 of sigma70 of E. coli RNA polymerase, polymer, 106 residues, 12164 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b
Entity Sequences (FASTA):
region 4 of sigma70 of E. coli RNA polymerase: GSSHHHHHHSSGLVPRGSHM
LELPLDSATTESLRAATHDV
LAGLTAREAKVLRMRFGIDM
NTDYTLEEVGKQFDVTRERI
RQIEAKALRKLRHPSRSEVL
RSFLDD
- assigned_chemical_shifts
- coupling_constants
- heteronucl_T1_relaxation
- heteronucl_T2_relaxation
- heteronucl_NOEs
| Data type | Count |
| 1H chemical shifts | 681 |
| 13C chemical shifts | 435 |
| 15N chemical shifts | 108 |
| coupling constants | 66 |
| T1 relaxation values | 94 |
| T2 relaxation values | 94 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 4 sigma70 | 1 |
Entities:
Entity 1, 4 sigma70 106 residues - 12164 Da.
| 1 | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | SER | ||||
| 2 | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | MET | ||||
| 3 | LEU | GLU | LEU | PRO | LEU | ASP | SER | ALA | THR | THR | ||||
| 4 | GLU | SER | LEU | ARG | ALA | ALA | THR | HIS | ASP | VAL | ||||
| 5 | LEU | ALA | GLY | LEU | THR | ALA | ARG | GLU | ALA | LYS | ||||
| 6 | VAL | LEU | ARG | MET | ARG | PHE | GLY | ILE | ASP | MET | ||||
| 7 | ASN | THR | ASP | TYR | THR | LEU | GLU | GLU | VAL | GLY | ||||
| 8 | LYS | GLN | PHE | ASP | VAL | THR | ARG | GLU | ARG | ILE | ||||
| 9 | ARG | GLN | ILE | GLU | ALA | LYS | ALA | LEU | ARG | LYS | ||||
| 10 | LEU | ARG | HIS | PRO | SER | ARG | SER | GLU | VAL | LEU | ||||
| 11 | ARG | SER | PHE | LEU | ASP | ASP |
Samples:
sample_1: region 4 of sigma70 of E. coli RNA polymerase, [U-13C; U-15N], 3 mM
cond_1: pH: 2.8; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | not available | not available | not available |
| 2D 1H-13C HSQC | not available | not available | not available |
| 3D C(CO)NH | not available | not available | not available |
| 3D CBCA(CO)NH | not available | not available | not available |
| 3D CBCANH | not available | not available | not available |
| 3D HNCO | not available | not available | not available |
| 3D (HCA)CO(CA)NH | not available | not available | not available |
| 2D J-MODULATED 1H-15N HSQC | not available | not available | not available |
| 2D H(N)CO | not available | not available | not available |
| 3D HA(CO)NH | not available | not available | not available |
| 3D H(CO)NH | not available | not available | not available |
| 3D 1H-1H-15N NOESY | not available | not available | not available |
Software:
nmrpipe v1.7 - data processing
XEasy v1.3.11 - peak assignment, sequential assignment
NMR spectrometers:
- Varian Unity Plus 500 MHz
Related Database Links:
| BMRB | 15930 15936 15975 |
| PDB | |
| DBJ | BAB37373 BAE77118 BAG66772 BAG78873 BAH65247 |
| EMBL | CAD07736 CAL34114 CAP77541 CAQ33404 CAQ90501 |
| GB | AAA24601 AAA27242 AAA89147 AAB60181 AAC76103 |
| PIR | AB0893 |
| REF | NP_289642 NP_311977 NP_417539 NP_457602 NP_462126 |
| SP | P00579 P0A2E3 P0A2E4 |
| AlphaFold | P0A2E3 P00579 P0A2E4 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts