BMRB Entry 4874
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4874
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Title: Sequence-specific assignment of the PAH2 domain of Sin3B free and complexed to Mad1
Deposition date: 2000-10-23 Original release date: 2001-04-26
Authors: Spronk, Christian; Jansen, Jacobus; Tessari, Marco; Kaan, Anita; Aelen, Jan; Vermeulen, Michiel; Stunnenberg, Hendrik; Vuister, Geerten
Citation: Spronk, Christian; Jansen, Jacobus; Tessari, Marco; Kaan, Anita; Aelen, Jan; Lasonder, Edwin; Stunnenberg, Hendrik; Vuister, Geerten. "Letter to the editor: Sequence-specific assignment of the PAH2 domain of Sin3B free and bound to Mad1" J. Biomol. NMR 19, 377-378 (2001).
Assembly members:
mSin3B-PAH2 domain, polymer, 105 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX2T
Entity Sequences (FASTA):
mSin3B-PAH2 domain: ESDSVEFNNAISYVNKIKTR
FLDHPEIYRSFLEILHTYQK
EQLHTKGRPFRGMSEEEVFT
EVANLFRGQEDLLSEFGQFL
PEAKRSLFTGNGSAEMNSGQ
KNEEK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 296 |
| 1H chemical shifts | 102 |
| 15N chemical shifts | 102 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PAH2 | 1 |
Entities:
Entity 1, PAH2 105 residues - Formula weight is not available
| 1 | GLU | SER | ASP | SER | VAL | GLU | PHE | ASN | ASN | ALA | ||||
| 2 | ILE | SER | TYR | VAL | ASN | LYS | ILE | LYS | THR | ARG | ||||
| 3 | PHE | LEU | ASP | HIS | PRO | GLU | ILE | TYR | ARG | SER | ||||
| 4 | PHE | LEU | GLU | ILE | LEU | HIS | THR | TYR | GLN | LYS | ||||
| 5 | GLU | GLN | LEU | HIS | THR | LYS | GLY | ARG | PRO | PHE | ||||
| 6 | ARG | GLY | MET | SER | GLU | GLU | GLU | VAL | PHE | THR | ||||
| 7 | GLU | VAL | ALA | ASN | LEU | PHE | ARG | GLY | GLN | GLU | ||||
| 8 | ASP | LEU | LEU | SER | GLU | PHE | GLY | GLN | PHE | LEU | ||||
| 9 | PRO | GLU | ALA | LYS | ARG | SER | LEU | PHE | THR | GLY | ||||
| 10 | ASN | GLY | SER | ALA | GLU | MET | ASN | SER | GLY | GLN | ||||
| 11 | LYS | ASN | GLU | GLU | LYS |
Samples:
Typical_sample: mSin3B-PAH2 domain, [U-100% 13C; U-100% 15N], 1.0 mM; Potassium Phosphate 50 mM; Potassium Chloride 100 mM
sample_conditions: pH: 6.5 na; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | Typical_sample | not available | sample_conditions |
| HNCO | Typical_sample | not available | sample_conditions |
| CBCA(CO)NNH | Typical_sample | not available | sample_conditions |
| HNCACB | Typical_sample | not available | sample_conditions |
Software:
NMRPipe v1.7 - Data processing
XEASY v1.3.9 - Spectral analysis
NMR spectrometers:
- Varian Inova 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts