BMRB Entry 4934
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4934
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Title: Structure and functionality of a designed p53 dimer
Deposition date: 2000-12-21 Original release date: 2001-01-23
Authors: Davison, T.; Nie, X.; Ma, W.; Lin, Y.; Kay, C.; Benchimol, S.; Arrowsmith, Cheryl
Citation: Davison, T.; Nie, X.; Ma, W.; Lin, Y.; Kay, C.; Benchimol, S.; Arrowsmith, Cheryl. "Structure and functionality of a designed p53 dimer" J. Mol. Biol. ., .-..
Assembly members:
p53, polymer, 33 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapien
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
p53: DGEYFTLQIRGRERFEQFRE
RNEALELKDAQAG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 95 |
| 15N chemical shifts | 37 |
| 1H chemical shifts | 228 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | p53 subunit 1 | 1 |
| 2 | p53 subunit 2 | 1 |
Entities:
Entity 1, p53 subunit 1 33 residues - Formula weight is not available
| 1 | ASP | GLY | GLU | TYR | PHE | THR | LEU | GLN | ILE | ARG | ||||
| 2 | GLY | ARG | GLU | ARG | PHE | GLU | GLN | PHE | ARG | GLU | ||||
| 3 | ARG | ASN | GLU | ALA | LEU | GLU | LEU | LYS | ASP | ALA | ||||
| 4 | GLN | ALA | GLY |
Samples:
sample_1: p53, [U-15N; U-13C], 1.85 – 2.1 mM; D2O 10%
Ex-cond_1: ionic strength: 0.125 M; pH: 7.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY | not available | not available | not available |
| HNCA | not available | not available | not available |
| HNCACB | not available | not available | not available |
| (HB)CBCA(CO)NNH | not available | not available | not available |
| N15-NOESY-HSQC | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| CCC-TOCSY | not available | not available | not available |
| 13C-HSQC | not available | not available | not available |
| 15N TOCSY HSQC | not available | not available | not available |
| HNHA | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Varian Unity Plus 500 MHz
- Varian Unity Plus 600 MHz
Related Database Links:
| PDB |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
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