BMRB Entry 4936

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4936

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N chemical shift assignments of the outer membrane protein OmpX from E.coli in DHPC micelles

Deposition date:

2001-01-09

Original release date:

2005-09-22

Authors:

Fernandez, Cesar; Adeishvili, Koba; Wuthrich, Kurt

Citation:

Citation: Fernandez, Cesar; Adeishvili, Koba; Wuthrich, Kurt. "Transverse relaxation-optimized NMR spectroscopy with the outer membrane protein OmpX in dihexanoyl phosphatidylcholine micelles." Proc. Natl. Acad. Sci. U. S. A. 98, 2358-2363 (2001).
PubMed: 11226244

Assembly members:

Assembly members:
Outer membrane protein X, polymer, 148 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Escherichia coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3b-OmpX

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Outer membrane protein X: ATSTVTGGYAQSDAQGQMNK MGGFNLKYRYEEDNSPLGVI GSFTYTEKSRTASSGDYNKN QYYGITAGPAYRINDWASIY GVVGVGYGKFQTTEYPTYKH DTTDYGFSYGAGLQFNPMEN VALDFSYEQSRIRSVDVGTW IAGVGYRF

Data sets:

assigned_chemical_shifts
- chemical_shifts

Data type	Count
1H chemical shifts	140
13C chemical shifts	407
15N chemical shifts	140

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	OmpX	1

Entities:

Entity 1, OmpX 148 residues - Formula weight is not available

1

ALA

THR

SER

THR

VAL

THR

GLY

TYR

ALA

2

GLN

SER

ASP

ALA

GLN

GLY

GLN

MET

ASN

LYS

3

MET

GLY

PHE

ASN

LEU

LYS

TYR

ARG

TYR

4

GLU

ASP

ASN

SER

PRO

LEU

GLY

VAL

ILE

5

GLY

SER

PHE

THR

TYR

THR

GLU

LYS

SER

ARG

6

THR

ALA

SER

GLY

ASP

TYR

ASN

LYS

ASN

7

GLN

TYR

GLY

ILE

THR

ALA

GLY

PRO

ALA

8

TYR

ARG

ILE

ASN

ASP

TRP

ALA

SER

ILE

TYR

9

GLY

VAL

GLY

VAL

GLY

TYR

GLY

LYS

PHE

10

GLN

THR

GLU

TYR

PRO

THR

TYR

LYS

HIS

11

ASP

THR

ASP

TYR

GLY

PHE

SER

TYR

GLY

12

ALA

GLY

LEU

GLN

PHE

ASN

PRO

MET

GLU

ASN

13

VAL

ALA

LEU

ASP

PHE

SER

TYR

GLU

GLN

SER

14

ARG

ILE

ARG

SER

VAL

ASP

VAL

GLY

THR

TRP

15

ILE

ALA

GLY

VAL

GLY

TYR

ARG

PHE

Samples:

sample: Outer membrane protein X, [U-98% 13C; U-98% 15N; U-2H], 2.0 mM; DHPC 300 mM; H2O 97%; D2O 3%; KPO4 20 mM; NaCl 100 mM

exp_conditions: pH: 6.8; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCA	sample	not available	exp_conditions
HN(CO)CA	sample	not available	exp_conditions
HNCACB	sample	not available	exp_conditions
3D [1H,1H]-NOESY-[15N,1H]-TROSY	sample	not available	exp_conditions

Software:

XEASY -

NMR spectrometers:

Bruker DRX 750 MHz

BMRB	15201 18796 18797
PDB	1ORM 1Q9F 1Q9G 1QJ8 1QJ9 2M06 2M07
DBJ	BAA35486 BAB34315 BAG76394 BAI24257 BAI29701
EMBL	CAP75284 CAQ31315 CAQ97717 CAR02170 CAR06985
GB	AAA21856 AAA66329 AAC73901 AAG55186 AAN42399
REF	NP_286578 NP_308919 NP_415335 NP_706692 NP_752830
SP	P0A917 P0A918 P0A919 P0A920
AlphaFold	P0A917 P0A918 P0A919 P0A920