BMRB Entry 4937
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4937
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Title: 1H chemical shifts for 'Hairpinless' mutant of omega-atracotoxin-Hv1a
Deposition date: 2001-01-10 Original release date: 2001-01-23
Authors: Fletcher, Jamie; King, Glenn
Citation: Tedford, Hugo; Fletcher, Jamie; King, Glenn. "Functional significance of the beta-hairpin in the insecticidal neurotoxin omega-atracotoxin-Hv1a" .
Assembly members:
Hairpinless mutant of omega-atracotoxin-Hv1a, polymer, 25 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: 6904 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Hadronyche versuta
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Hairpinless mutant of omega-atracotoxin-Hv1a: CIPSGQPCPYNENCCSQSCT
GGRCD
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 127 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hairpinless monomer | 1 |
Entities:
Entity 1, hairpinless monomer 25 residues - Formula weight is not available
| 1 | CYS | ILE | PRO | SER | GLY | GLN | PRO | CYS | PRO | TYR | ||||
| 2 | ASN | GLU | ASN | CYS | CYS | SER | GLN | SER | CYS | THR | ||||
| 3 | GLY | GLY | ARG | CYS | ASP |
Samples:
Sample_1: Hairpinless mutant of omega-atracotoxin-Hv1a 3 mM; chloramphenicol 25 uM; TSP 0.1 mM
Ex-cond_1: ionic strength: 0.005 M; pH: 4.9; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | Sample_1 | not available | Ex-cond_1 |
| 2D 1H-1H TOCSY | Sample_1 | not available | Ex-cond_1 |
| 2D 1H-1H DQF-COSY | Sample_1 | not available | Ex-cond_1 |
| 2D 1H-1H E.COSY | Sample_1 | not available | Ex-cond_1 |
Software:
xwinnmr v2.0 - data acquisition, data processing
XEASY v1.3.13 - data acquisition, data processing, peak integration, spectral analysis
NMR spectrometers:
- Bruker DRX 600 MHz