BMRB Entry 4958
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4958
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Title: 1H, 13C and 15N Sequence-specific Resonance Assignment of the PSCD4 Domain of Diatom cell wall Protein Pleuralin-1
Deposition date: 2001-02-20 Original release date: 2002-04-03
Authors: Wenzler, Michael; Brunner, Eike; Kroger, Nils; Lehmann, Gerhard; Sumper, Manfred; Kalbitzer, Hans; De Sanctis, Silvia; Malloni, Wilhelm
Citation: Wenzler, Michael; Brunner, Eike; Kroger, Nils; Lehmann, Gerhard; Sumper, Manfred; Kalbitzer, Hans. "Letter to the Editor: 1H, 13C and 15N Sequence-specific Resonance Assignment of the PSCD4 Domain of Diatom cell wall Protein Pleuralin-1" J. Biomol. NMR 20, 191-192 (2001).
Assembly members:
pleuralin-1, formerly HEP200, polymer, 112 residues, 11946 Da.
Natural source: Common Name: Cylindrotheca fusiformis Taxonomy ID: 2853 Superkingdom: Eukaryota Kingdom: not available Genus/species: Cylindrotheca fusiformis
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
pleuralin-1, formerly HEP200: SYYHHHHHHTMMPSPEPSSQ
PSDCGEVIEECPIDACFLPK
SDSARPPDCTAVGRPDCNVL
PFPNNIGCPSCCPFECSPDN
PMFTPSPDGSPPNCSPTMLP
SPSPSAVTVPLT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 391 |
| 15N chemical shifts | 86 |
| 1H chemical shifts | 597 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | His6PSCD4 | 1 |
Entities:
Entity 1, His6PSCD4 112 residues - 11946 Da.
| 1 | SER | TYR | TYR | HIS | HIS | HIS | HIS | HIS | HIS | THR | ||||
| 2 | MET | MET | PRO | SER | PRO | GLU | PRO | SER | SER | GLN | ||||
| 3 | PRO | SER | ASP | CYS | GLY | GLU | VAL | ILE | GLU | GLU | ||||
| 4 | CYS | PRO | ILE | ASP | ALA | CYS | PHE | LEU | PRO | LYS | ||||
| 5 | SER | ASP | SER | ALA | ARG | PRO | PRO | ASP | CYS | THR | ||||
| 6 | ALA | VAL | GLY | ARG | PRO | ASP | CYS | ASN | VAL | LEU | ||||
| 7 | PRO | PHE | PRO | ASN | ASN | ILE | GLY | CYS | PRO | SER | ||||
| 8 | CYS | CYS | PRO | PHE | GLU | CYS | SER | PRO | ASP | ASN | ||||
| 9 | PRO | MET | PHE | THR | PRO | SER | PRO | ASP | GLY | SER | ||||
| 10 | PRO | PRO | ASN | CYS | SER | PRO | THR | MET | LEU | PRO | ||||
| 11 | SER | PRO | SER | PRO | SER | ALA | VAL | THR | VAL | PRO | ||||
| 12 | LEU | THR |
Samples:
unlabeled: pleuralin-1, formerly HEP200 0.8 mM
15N_labeled: pleuralin-1, formerly HEP200, [U-97% 15N], 0.8 mM
13C_15N_labeled: pleuralin-1, formerly HEP200, [U-97% 13C; U-98% 15N], 0.8 mM
Ex-cond_1: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCA | not available | not available | Ex-cond_1 |
| CBCA(CO)NH | not available | not available | Ex-cond_1 |
| 1H-15N-HSQC | not available | not available | Ex-cond_1 |
| 1H-15N-NOESY HSQC | not available | not available | Ex-cond_1 |
| 1H-15N-TOCSY HSQC | not available | not available | Ex-cond_1 |
| HNCO | not available | not available | Ex-cond_1 |
| HACACO | not available | not available | Ex-cond_1 |
| HCCH-TOCSY | not available | not available | Ex-cond_1 |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DRX 800 MHz
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or all simulated shifts
SPARKY: Backbone
or all simulated shifts