BMRB Entry 4981
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4981
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Title: Identification of the phospholipid binding site of human beta2-glycoprotein I domain V by heteronuclear magnetic resonance PubMed: 11124037
Deposition date: 2001-03-27 Original release date: 2001-05-29
Authors: Hoshino, Masaru; Hagihara, Yoshihisa; Nishii, Ichiro; Yamazaki, Toshio; Kato, Hisao; Goto, Yuji
Citation: Hoshino, Masaru; Hagihara, Yoshihisa; Nishii, Ichiro; Yamazaki, Toshio; Kato, Hisao; Goto, Yuji. "Identification of the phospholipid binding site of human beta2-glycoprotein I domain V by heteronuclear magnetic resonance" J. Mol. Biol. 304, 927-940 (2000).
Assembly members:
beta2-glycoprotein I, polymer, 86 residues, Formula weight is not available
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Pichia pastoris
Entity Sequences (FASTA):
beta2-glycoprotein I: TKASCKLPVKKATVVYQGER
VKIQEKFKNGMLHGDKVSFF
CKNKEKKCSYTEDAQCIDGT
IEVPKCFKEHSSLAFWKTDA
SDVKPC
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 610 |
| 13C chemical shifts | 389 |
| 15N chemical shifts | 88 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | beta2-GPI domain V | 1 |
Entities:
Entity 1, beta2-GPI domain V 86 residues - Formula weight is not available
| 1 | THR | LYS | ALA | SER | CYS | LYS | LEU | PRO | VAL | LYS | ||||
| 2 | LYS | ALA | THR | VAL | VAL | TYR | GLN | GLY | GLU | ARG | ||||
| 3 | VAL | LYS | ILE | GLN | GLU | LYS | PHE | LYS | ASN | GLY | ||||
| 4 | MET | LEU | HIS | GLY | ASP | LYS | VAL | SER | PHE | PHE | ||||
| 5 | CYS | LYS | ASN | LYS | GLU | LYS | LYS | CYS | SER | TYR | ||||
| 6 | THR | GLU | ASP | ALA | GLN | CYS | ILE | ASP | GLY | THR | ||||
| 7 | ILE | GLU | VAL | PRO | LYS | CYS | PHE | LYS | GLU | HIS | ||||
| 8 | SER | SER | LEU | ALA | PHE | TRP | LYS | THR | ASP | ALA | ||||
| 9 | SER | ASP | VAL | LYS | PRO | CYS |
Samples:
sample_1: beta2-glycoprotein I, [U-15N], 1.0 mM
sample_2: beta2-glycoprotein I, [U-15N; U-13C], 1.0 mM
condition_1: pH: 6.0; temperature: 298 K; ionic strength: 0.12 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HNCACB | not available | not available | condition_1 |
| CBCA(CO)NH | not available | not available | condition_1 |
| HN(CO)CAHA | not available | not available | condition_1 |
| 1H-13C-1H HCCH-TOCSY | not available | not available | condition_1 |
| 1H-1H-15N NOESY | not available | not available | condition_1 |
| 13C-1H-1H NOESY | not available | not available | condition_1 |
| HNCO | not available | not available | condition_1 |
Software:
nmrPipe v1.7 - processing
pipp v3.9.9 - processing
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DRX 800 MHz
Related Database Links:
| BMRB | 16639 |
| PDB | |
| DBJ | BAG36570 BAG60642 BAI46022 |
| EMBL | CAA37664 CAA40977 CAA41113 CAA72279 |
| GB | AAA51766 AAB21330 AAH20703 AAH26283 AAK71538 |
| REF | NP_000033 NP_001009118 XP_001084366 XP_002748690 XP_002827786 |
| SP | P02749 Q95LB0 |
| AlphaFold | Q95LB0 P02749 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
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SPARKY: Backbone
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