BMRB Entry 50002
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50002
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Title: Pof8 RNA recognition motif 2 PubMed: 33131423
Deposition date: 2019-08-18 Original release date: 2021-08-13
Authors: Eichhorn, Catherine; Feigon, Juli
Citation: Basu, Ritwika; Eichhorn, Catherine; Cheng, Ryan; Peterson, Robert; Feigon, Juli. "Structure of S. pombe telomerase protein Pof8 C-terminal domain is an xRRM conserved among LARP7 proteins" RNA Biol. 18, 1181-1192 (2021).
Assembly members:
entity_1, polymer, 121 residues, Formula weight is not available
Natural source: Common Name: fission yeast Taxonomy ID: 4896 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Schizosaccharomyces pombe
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET
Entity Sequences (FASTA):
entity_1: KDEDNLDFTKNLLTRIKNLH
PLTNKSTIHSLLSYVFSRQT
QNIACEPMYIDYRKDETEAI
IRWKTPLHAETCINAFRTQE
RKQNSHDDIRAHRKKGSSRP
FLIAELITGEEEKNYWRMLK
K
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 276 |
| 15N chemical shifts | 106 |
| 1H chemical shifts | 131 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Pof8 C-terminal xRRM | 1 |
Entities:
Entity 1, Pof8 C-terminal xRRM 121 residues - Formula weight is not available
| 1 | LYS | ASP | GLU | ASP | ASN | LEU | ASP | PHE | THR | LYS | ||||
| 2 | ASN | LEU | LEU | THR | ARG | ILE | LYS | ASN | LEU | HIS | ||||
| 3 | PRO | LEU | THR | ASN | LYS | SER | THR | ILE | HIS | SER | ||||
| 4 | LEU | LEU | SER | TYR | VAL | PHE | SER | ARG | GLN | THR | ||||
| 5 | GLN | ASN | ILE | ALA | CYS | GLU | PRO | MET | TYR | ILE | ||||
| 6 | ASP | TYR | ARG | LYS | ASP | GLU | THR | GLU | ALA | ILE | ||||
| 7 | ILE | ARG | TRP | LYS | THR | PRO | LEU | HIS | ALA | GLU | ||||
| 8 | THR | CYS | ILE | ASN | ALA | PHE | ARG | THR | GLN | GLU | ||||
| 9 | ARG | LYS | GLN | ASN | SER | HIS | ASP | ASP | ILE | ARG | ||||
| 10 | ALA | HIS | ARG | LYS | LYS | GLY | SER | SER | ARG | PRO | ||||
| 11 | PHE | LEU | ILE | ALA | GLU | LEU | ILE | THR | GLY | GLU | ||||
| 12 | GLU | GLU | LYS | ASN | TYR | TRP | ARG | MET | LEU | LYS | ||||
| 13 | LYS |
Samples:
sample_1: Pof8 C-terminal xRRM, [U-13C; U-15N], 0.8 mM; sodium phosphate 20 mM; potassium chloride 50 mM; TCEP 1 mM
sample_conditions_1: ionic strength: 50 mM; pH: 6.1; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 15N-(1H) NOE | sample_1 | isotropic | sample_conditions_1 |
Software:
SPARKY, Goddard - chemical shift assignment, data analysis
TOPSPIN, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment
NMR spectrometers:
- Bruker DRX 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts