BMRB Entry 50009

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50009

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Title:
Backbone and methyl assignment of bacteriorhodopsin incorporated into nanodiscs
Deposition date:
2019-09-07
Original release date:
2020-02-24
Authors:
Kooijman, Laurens; Ansorge, Philipp; Schuster, Matthias; Baumann, Christian; Lohr, Frank; Jurt, Simon; Guntert, Peter; Zerbe, Oliver
Citation:

Citation: Kooijman, Laurens; Ansorge, Philipp; Schuster, Matthias; Baumann, Christian; Lohr, Frank; Jurt, Simon; Guntert, Peter; Zerbe, Oliver. "Backbone and methyl assignment of bacteriorhodopsin incorporated into nanodiscs"  J. Biomol. NMR. 74, 45-60 (2020).
PubMed: 31754899

Assembly members:

Assembly members:
entity_1, polymer, 259 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Halobacterium halobium   Taxonomy ID: 2242   Superkingdom: Archaea   Kingdom: not available   Genus/species: Halobacterium halobium

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSKLAQITGRPEWIWLALGT ALMGLGTLYFLVKGMGVSDP DAKKFYAITTLVPAIAFTMY LSMLLGYGLTMVPFGGEQNP IYWARYADWLFTTPLLLLDL ALLVDADQGTILALVGADGI MIGTGLVGALTKVYSYRFVW WAISTAAMLYILYVLFFGFT SKAESMRPEVASTFKVLRNV TVVLWSAYPVVWLIGSEGAG IVPLNIETLLFMVLDVSAKV GFGLILLRSRAIFGEAEAPE PSAGDGAAATSLEHHHHHH

Data sets:
Data typeCount
13C chemical shifts515
15N chemical shifts156
1H chemical shifts465

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Bacteriorhodopsin1

Entities:

Entity 1, Bacteriorhodopsin 259 residues - Formula weight is not available

1   GLYSERLYSLEUALAGLNILETHRGLYARG
2   PROGLUTRPILETRPLEUALALEUGLYTHR
3   ALALEUMETGLYLEUGLYTHRLEUTYRPHE
4   LEUVALLYSGLYMETGLYVALSERASPPRO
5   ASPALALYSLYSPHETYRALAILETHRTHR
6   LEUVALPROALAILEALAPHETHRMETTYR
7   LEUSERMETLEULEUGLYTYRGLYLEUTHR
8   METVALPROPHEGLYGLYGLUGLNASNPRO
9   ILETYRTRPALAARGTYRALAASPTRPLEU
10   PHETHRTHRPROLEULEULEULEUASPLEU
11   ALALEULEUVALASPALAASPGLNGLYTHR
12   ILELEUALALEUVALGLYALAASPGLYILE
13   METILEGLYTHRGLYLEUVALGLYALALEU
14   THRLYSVALTYRSERTYRARGPHEVALTRP
15   TRPALAILESERTHRALAALAMETLEUTYR
16   ILELEUTYRVALLEUPHEPHEGLYPHETHR
17   SERLYSALAGLUSERMETARGPROGLUVAL
18   ALASERTHRPHELYSVALLEUARGASNVAL
19   THRVALVALLEUTRPSERALATYRPROVAL
20   VALTRPLEUILEGLYSERGLUGLYALAGLY
21   ILEVALPROLEUASNILEGLUTHRLEULEU
22   PHEMETVALLEUASPVALSERALALYSVAL
23   GLYPHEGLYLEUILELEULEUARGSERARG
24   ALAILEPHEGLYGLUALAGLUALAPROGLU
25   PROSERALAGLYASPGLYALAALAALATHR
26   SERLEUGLUHISHISHISHISHISHIS

Samples:

sample_1: Bacteriorhodopsin, [U-2H; U-15N; U-13; ILV 13C/1H], 350 uM; potassium phosphate 40 mM

sample_2: Bacteriorhodopsin, [U-2H; U-15N; U-13; AILV 13C/1H], 500 uM; potassium phosphate 40 mM

sample_3: Bacteriorhodopsin, [U-2H; U-15N; U-13; MILV 13C/1H], 450 uM; potassium phosphate 40 mM

sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 320 K

Experiments:

NameSampleSample stateSample conditions
NAsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 900 MHz
  • Bruker Avance 950 MHz

Related Database Links:

SP P02945
AlphaFold Q9HPU5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks