BMRB Entry 50009
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50009
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Title: Backbone and methyl assignment of bacteriorhodopsin incorporated into nanodiscs PubMed: 31754899
Deposition date: 2019-09-07 Original release date: 2020-02-24
Authors: Kooijman, Laurens; Ansorge, Philipp; Schuster, Matthias; Baumann, Christian; Lohr, Frank; Jurt, Simon; Guntert, Peter; Zerbe, Oliver
Citation: Kooijman, Laurens; Ansorge, Philipp; Schuster, Matthias; Baumann, Christian; Lohr, Frank; Jurt, Simon; Guntert, Peter; Zerbe, Oliver. "Backbone and methyl assignment of bacteriorhodopsin incorporated into nanodiscs" J. Biomol. NMR. 74, 45-60 (2020).
Assembly members:
entity_1, polymer, 259 residues, Formula weight is not available
Natural source: Common Name: Halobacterium halobium Taxonomy ID: 2242 Superkingdom: Archaea Kingdom: not available Genus/species: Halobacterium halobium
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28
Entity Sequences (FASTA):
entity_1: GSKLAQITGRPEWIWLALGT
ALMGLGTLYFLVKGMGVSDP
DAKKFYAITTLVPAIAFTMY
LSMLLGYGLTMVPFGGEQNP
IYWARYADWLFTTPLLLLDL
ALLVDADQGTILALVGADGI
MIGTGLVGALTKVYSYRFVW
WAISTAAMLYILYVLFFGFT
SKAESMRPEVASTFKVLRNV
TVVLWSAYPVVWLIGSEGAG
IVPLNIETLLFMVLDVSAKV
GFGLILLRSRAIFGEAEAPE
PSAGDGAAATSLEHHHHHH
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 515 |
15N chemical shifts | 156 |
1H chemical shifts | 465 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Bacteriorhodopsin | 1 |
Entities:
Entity 1, Bacteriorhodopsin 259 residues - Formula weight is not available
1 | GLY | SER | LYS | LEU | ALA | GLN | ILE | THR | GLY | ARG | ||||
2 | PRO | GLU | TRP | ILE | TRP | LEU | ALA | LEU | GLY | THR | ||||
3 | ALA | LEU | MET | GLY | LEU | GLY | THR | LEU | TYR | PHE | ||||
4 | LEU | VAL | LYS | GLY | MET | GLY | VAL | SER | ASP | PRO | ||||
5 | ASP | ALA | LYS | LYS | PHE | TYR | ALA | ILE | THR | THR | ||||
6 | LEU | VAL | PRO | ALA | ILE | ALA | PHE | THR | MET | TYR | ||||
7 | LEU | SER | MET | LEU | LEU | GLY | TYR | GLY | LEU | THR | ||||
8 | MET | VAL | PRO | PHE | GLY | GLY | GLU | GLN | ASN | PRO | ||||
9 | ILE | TYR | TRP | ALA | ARG | TYR | ALA | ASP | TRP | LEU | ||||
10 | PHE | THR | THR | PRO | LEU | LEU | LEU | LEU | ASP | LEU | ||||
11 | ALA | LEU | LEU | VAL | ASP | ALA | ASP | GLN | GLY | THR | ||||
12 | ILE | LEU | ALA | LEU | VAL | GLY | ALA | ASP | GLY | ILE | ||||
13 | MET | ILE | GLY | THR | GLY | LEU | VAL | GLY | ALA | LEU | ||||
14 | THR | LYS | VAL | TYR | SER | TYR | ARG | PHE | VAL | TRP | ||||
15 | TRP | ALA | ILE | SER | THR | ALA | ALA | MET | LEU | TYR | ||||
16 | ILE | LEU | TYR | VAL | LEU | PHE | PHE | GLY | PHE | THR | ||||
17 | SER | LYS | ALA | GLU | SER | MET | ARG | PRO | GLU | VAL | ||||
18 | ALA | SER | THR | PHE | LYS | VAL | LEU | ARG | ASN | VAL | ||||
19 | THR | VAL | VAL | LEU | TRP | SER | ALA | TYR | PRO | VAL | ||||
20 | VAL | TRP | LEU | ILE | GLY | SER | GLU | GLY | ALA | GLY | ||||
21 | ILE | VAL | PRO | LEU | ASN | ILE | GLU | THR | LEU | LEU | ||||
22 | PHE | MET | VAL | LEU | ASP | VAL | SER | ALA | LYS | VAL | ||||
23 | GLY | PHE | GLY | LEU | ILE | LEU | LEU | ARG | SER | ARG | ||||
24 | ALA | ILE | PHE | GLY | GLU | ALA | GLU | ALA | PRO | GLU | ||||
25 | PRO | SER | ALA | GLY | ASP | GLY | ALA | ALA | ALA | THR | ||||
26 | SER | LEU | GLU | HIS | HIS | HIS | HIS | HIS | HIS |
Samples:
sample_1: Bacteriorhodopsin, [U-2H; U-15N; U-13; ILV 13C/1H], 350 uM; potassium phosphate 40 mM
sample_2: Bacteriorhodopsin, [U-2H; U-15N; U-13; AILV 13C/1H], 500 uM; potassium phosphate 40 mM
sample_3: Bacteriorhodopsin, [U-2H; U-15N; U-13; MILV 13C/1H], 450 uM; potassium phosphate 40 mM
sample_conditions_1: pH: 6.4; pressure: 1 atm; temperature: 320 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
NA | sample_1 | isotropic | sample_conditions_1 |
Software:
CYANA, Guntert, Mumenthaler and Wuthrich - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz
- Bruker Avance 800 MHz
- Bruker Avance 900 MHz
- Bruker Avance 950 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts