BMRB Entry 50014
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50014
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Title: ctSpp2(208-254) backbone assignment PubMed: 31974312
Deposition date: 2019-09-12 Original release date: 2020-09-21
Authors: Favretto, Filippo; Zweckstetter, Markus
Citation: Hamann, Florian; Schmitt, Andreas; Favretto, Filippo; Hofele, Romina; Neumann, Piotr; Xiang, ShengQi; Urlaub, Henning; Zweckstetter, Markus; Ficner, Ralf. "Structural analysis of the intrinsically disordered splicing factor Spp2 and its binding to the DEAH-box ATPase Prp2" Proc. Natl. Acad. Sci. U.S.A. 117, 2948-2956 (2020).
Assembly members:
Molecule 1, polymer, 47 residues, 5226.9257 Da.
Natural source: Common Name: not available Taxonomy ID: 209285 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Chaetomium thermophilum
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pASG-IBA 25
Entity Sequences (FASTA):
Molecule 1: GPMVDDFGENLLRSFGWDGK
MRGKVKEVKRYANLAGLGAR
NVKEAED
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 13C chemical shifts | 130 |
| 15N chemical shifts | 45 |
| 1H chemical shifts | 45 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Molecule 1 | 1 |
Entities:
Entity 1, Molecule 1 47 residues - 5226.9257 Da.
| 1 | GLY | PRO | MET | VAL | ASP | ASP | PHE | GLY | GLU | ASN | ||||
| 2 | LEU | LEU | ARG | SER | PHE | GLY | TRP | ASP | GLY | LYS | ||||
| 3 | MET | ARG | GLY | LYS | VAL | LYS | GLU | VAL | LYS | ARG | ||||
| 4 | TYR | ALA | ASN | LEU | ALA | GLY | LEU | GLY | ALA | ARG | ||||
| 5 | ASN | VAL | LYS | GLU | ALA | GLU | ASP |
Samples:
sample_1: ctSpp2, [15N 13C], 0.52 mM; DTT 1 mM; EDTA 5 mM; HEPES 10 mM; sodium chloride 200 mM; glycerol 0.5 % w/v; sodium azide 0.02 % w/v
sample_conditions_1: ionic strength: 0.2 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC/HMQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNmr Analysis v2.2, CCPN - chemical shift assignments
DANGLE v1.1, University of Cambridge - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts