BMRB Entry 50021
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50021
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Title: Backbone NMR assignments of the Smurf2 WW1 domain PubMed: 31628949
Deposition date: 2019-09-23 Original release date: 2020-09-21
Authors: Wiesner, Silke
Citation: Ruetalo, Natalia; Anders, Samira; Stollmaier, Carsten; Jaeckl, Magnus; Schuetz-Stoffregen, Mira; Stefan, Nadine; Wolf, Christine; Wiesner, Silke. "The WW1 Domain Enhances Autoinhibition in Smurf Ubiquitin Ligases" J. Mol. Biol. 431, 4834-4847 (2019).
Assembly members:
Smurf2 WW1 domain, polymer, 41 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM-41
Entity Sequences (FASTA):
Smurf2 WW1 domain: GAMGNDLPDGWEERRTASGR
IQYLNHITRTTQWERPTRPA
S
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 66 |
15N chemical shifts | 29 |
1H chemical shifts | 29 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | WW1 | 1 |
Entities:
Entity 1, WW1 41 residues - Formula weight is not available
1 | GLY | ALA | MET | GLY | ASN | ASP | LEU | PRO | ASP | GLY | ||||
2 | TRP | GLU | GLU | ARG | ARG | THR | ALA | SER | GLY | ARG | ||||
3 | ILE | GLN | TYR | LEU | ASN | HIS | ILE | THR | ARG | THR | ||||
4 | THR | GLN | TRP | GLU | ARG | PRO | THR | ARG | PRO | ALA | ||||
5 | SER |
Samples:
sample_1: sodium phosphate 20 mM; sodium chloride 150 mM; DTT 1 mM; WW1, [U-100% 13C], 1 mM
sample_conditions_1: ionic strength: 0.5 M; pH: 7.5; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
XEASY, Bartels et al. - chemical shift assignment
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts