BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 50040

Title: 1H, 13C, and 15N Chemical Shift Assignments for hairpin RNA HP021   PubMed: 33707433

Deposition date: 2019-10-07 Original release date: 2021-02-02

Authors: Wang, Yanjiao; Han, Ge; Jiang, Xiuying; Yuwen, Tairan; Al-Hashimi, Hashim; Xue, Yi

Citation: Wang, Yanjiao; Han, Ge; Jiang, Xiuying; Yuwen, Tairan; Xue, Yi. "Chemical shift prediction of RNA imino groups: application toward characterizing RNA excited states"  Nat. Commun. 12, 1595-1595 (2021).

Assembly members:
hairpin RNA hp021, polymer, 30 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: in vitro transcription   Host organism: in vitro transcription

Entity Sequences (FASTA):
hairpin RNA hp021: GGGGGGAUGUUGCUUCGGUG AUAUCCCUCC

Data sets:
Data typeCount
15N chemical shifts18
1H chemical shifts18

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HP0211

Entities:

Entity 1, HP021 30 residues - Formula weight is not available

1   GGGGGGAUGU
2   UGCUUCGGUG
3   AUAUCCCUCC

Samples:

sample_1: RNA(30-mer) 3.45 mM; sodium phosphate 10 mM; EDTA 0.01 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 10 mM; pH: 6.4; pressure: 1 atm; temperature: 283.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HMQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

SPARKY, Goddard - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker AVANCE III HD 600MHz MHz
  • Bruker AVANCE III HD 800MHZ MHz