BMRB Entry 50107

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50107

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Title:
1H, 13C and 15N chemical shift assignments for VPS29
Deposition date:
2019-11-29
Original release date:
2020-10-16
Authors:
Owen, David; Neuhaus, David; Yang, Ji-Chun; Crawley-Snowdon, Harriet
Citation:

Citation: Crawley-Snowdon, Harriet; Yang, Ji-Chun; Zaccai, Nathan; Davis, Luther; Wartosch, Lena; Herman, Emily; Bright, Nicholas; Swarbrick, James; Collins, Brett; Jackson, Lauren; Seaman, Matthew; Luzio, J Paul; Dacks, Joel; Neuhaus, David; Owen, David. "Mechanism and evolution of the Zn-fingernail required for interaction of VARP with VPS29"  Nat. Commun. 11, 5031-5031 (2020).
PubMed: 33024112

Assembly members:

Assembly members:
entity_1, polymer, 192 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEXVPS29

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSPEFGTRDRMLVLVLGDLH IPHRCNSLPAKFKKLLVPGK IQHILCTGNLCTKESYDYLK TLAGDVHIVRGDFDENLNYP EQKVVTVGQFKIGLIHGHQV IPWGDMASLALLQRQFDVDI LISGHTHKFEAFEHENKFYI NPGSATGAYNALETNIIPSF VLMDIQASTVVTYVYQLIGD DVKVERIEYKKS

Data sets:
Data typeCount
13C chemical shifts519
15N chemical shifts177
1H chemical shifts1026

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1VPS291

Entities:

Entity 1, VPS29 192 residues - Formula weight is not available

residues -6 to 0 are a cloning artefact.

1   GLYSERPROGLUPHEGLYTHRARGASPARG
2   METLEUVALLEUVALLEUGLYASPLEUHIS
3   ILEPROHISARGCYSASNSERLEUPROALA
4   LYSPHELYSLYSLEULEUVALPROGLYLYS
5   ILEGLNHISILELEUCYSTHRGLYASNLEU
6   CYSTHRLYSGLUSERTYRASPTYRLEULYS
7   THRLEUALAGLYASPVALHISILEVALARG
8   GLYASPPHEASPGLUASNLEUASNTYRPRO
9   GLUGLNLYSVALVALTHRVALGLYGLNPHE
10   LYSILEGLYLEUILEHISGLYHISGLNVAL
11   ILEPROTRPGLYASPMETALASERLEUALA
12   LEULEUGLNARGGLNPHEASPVALASPILE
13   LEUILESERGLYHISTHRHISLYSPHEGLU
14   ALAPHEGLUHISGLUASNLYSPHETYRILE
15   ASNPROGLYSERALATHRGLYALATYRASN
16   ALALEUGLUTHRASNILEILEPROSERPHE
17   VALLEUMETASPILEGLNALASERTHRVAL
18   VALTHRTYRVALTYRGLNLEUILEGLYASP
19   ASPVALLYSVALGLUARGILEGLUTYRLYS
20   LYSSER

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