BMRB Entry 50108
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50108
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Citation: Crawley-Snowdon, Harriet; Yang, Ji-Chun; Zaccai, Nathan; Davis, Luther; Wartosch, Lena; Herman, Emily; Bright, Nicholas; Swarbrick, James; Collins, Brett; Jackson, Lauren; Seaman, Matthew; Luzio, J Paul; Dacks, Joel; Neuhaus, David; Owen, David. "Mechanism and evolution of the Zn-fingernail required for interaction of VARP with VPS29" Nat. Commun. 11, 5031-5031 (2020).
PubMed: 33024112
Assembly members:
entity_1, polymer, 61 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEXVARPfingernail2
Entity Sequences (FASTA):
entity_1: GPLGSTEEDLEDAEDTVSAA
DPEFCHPLCQCPKCAPAQKR
LAKVPASGLGVNVTSQDGSS
W
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 181 |
| 15N chemical shifts | 58 |
| 1H chemical shifts | 359 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | VARP 687-747 | 1 |
| 2 | zinc ion | 2 |
Entities:
Entity 1, VARP 687-747 61 residues - Formula weight is not available
| 1 | GLY | PRO | LEU | GLY | SER | THR | GLU | GLU | ASP | LEU | ||||
| 2 | GLU | ASP | ALA | GLU | ASP | THR | VAL | SER | ALA | ALA | ||||
| 3 | ASP | PRO | GLU | PHE | CYS | HIS | PRO | LEU | CYS | GLN | ||||
| 4 | CYS | PRO | LYS | CYS | ALA | PRO | ALA | GLN | LYS | ARG | ||||
| 5 | LEU | ALA | LYS | VAL | PRO | ALA | SER | GLY | LEU | GLY | ||||
| 6 | VAL | ASN | VAL | THR | SER | GLN | ASP | GLY | SER | SER | ||||
| 7 | TRP |
Entity 2, zinc ion - Zn - 65.409 Da.
| 1 | ZN |
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