BMRB Entry 50109
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50109
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Title: Nonexchangeable proton shifts for d(GTATGGCCATAC)2 PubMed: 35726678
Deposition date: 2019-11-30 Original release date: 2019-12-17
Authors: Lyon, Rhiannon; Southam, Hannah; Trevitt, Clare; Liao, Chunyan; El-Khamisy, Sherif; Poole, Robert
Citation: Lyon, Rhiannon; Southam, Hannah; Trevitt, Clare; Liao, Chunyan; El-Khamisy, Sherif; Poole, Robert; Williamson, Mike. "CORM-3 induces DNA damage through Ru(II) binding to DNA" Biochem. J. 479, 1429-1439 (2022).
Assembly members:
entity_1, polymer, 12 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: GTATGGCCATAC
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 83 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DNA strand, 1 | 1 |
| 2 | DNA strand, 2 | 1 |
Entities:
Entity 1, DNA strand, 1 12 residues - Formula weight is not available
| 1 | DG | DT | DA | DT | DG | DG | DC | DC | DA | DT | ||||
| 2 | DA | DC |
Samples:
sample_1: entity_1 5 mM; potassium phosphate 30 ± 1 mM
sample_conditions_1: pH: 7.8; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN v2, Bruker Biospin - NMR processing and display
NMR spectrometers:
- Bruker Avance 800 MHz