BMRB Entry 50138

Name: NMR insights into the pre-amyloid ensemble and secretion targeting of the curli subunit CsgA
Published: 2020-06-09

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50138

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR insights into the pre-amyloid ensemble and secretion targeting of the curli subunit CsgA

Deposition date:

2019-12-18

Original release date:

2020-06-09

Authors:

Sewell, Lee; Stylianou, Fisentzos; Xu, Yingqi; Taylor, Jon; Sefer, Lea; Matthews, Steve

Citation:

Citation: Sewell, Lee; Stylianou, Fisentzos; Xu, Yingqi; Taylor, Jon; Sefer, Lea; Matthews, Steve. "NMR Insights Into the Pre-Amyloid Ensemble and Secretion Targeting of the Curli Subunit CsgA" Sci. Rep. 10, 7896-7896 (2020).
PubMed: 32398666

Assembly members:

Assembly members:
entity_1, polymer, 134 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MGVVPQYGGGGNHGGGGNNS GPNSELNIYQYGGGNSALAL QTDARNSDLTITQHGGGNGA DVGQGSDDSSIDLTQRGFGN SATLDQWNGKNSEMTVKQFG GGNGAAVDQTASNSSVNVTQ VGFGNNATAHQYLQ

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	301
15N chemical shifts	117
1H chemical shifts	117

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Pre-fibrillar CsgA monomer	1

Entities:

Entity 1, Pre-fibrillar CsgA monomer 134 residues - Formula weight is not available

1

MET

GLY

VAL

PRO

GLN

TYR

GLY

2

GLY

ASN

HIS

GLY

ASN

SER

3

GLY

PRO

ASN

SER

GLU

LEU

ASN

ILE

TYR

GLN

4

TYR

GLY

ASN

SER

ALA

LEU

ALA

LEU

5

GLN

THR

ASP

ALA

ARG

ASN

SER

ASP

LEU

THR

6

ILE

THR

GLN

HIS

GLY

ASN

GLY

ALA

7

ASP

VAL

GLY

GLN

GLY

SER

ASP

SER

8

ILE

ASP

LEU

THR

GLN

ARG

GLY

PHE

GLY

ASN

9

SER

ALA

THR

LEU

ASP

GLN

TRP

ASN

GLY

LYS

10

ASN

SER

GLU

MET

THR

VAL

LYS

GLN

PHE

GLY

11

GLY

ASN

GLY

ALA

VAL

ASP

GLN

THR

12

ALA

SER

ASN

SER

VAL

ASN

VAL

THR

GLN

13

VAL

GLY

PHE

GLY

ASN

ALA

THR

ALA

HIS

14

GLN

TYR

LEU

GLN

Samples:

sample_1: entity_1, [U-13C; U-15N], mM; sodium phosphate 20 mM

sample_conditions_1: pH: 7.2; pressure: 1 atm; temperature: 283.2 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker Avance 950 MHz
Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks