BMRB Entry 50142

Name: Nb-SUMO1
Published: 2020-08-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50142

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Nb-SUMO1

Deposition date:

2019-12-30

Original release date:

2020-08-14

Authors:

Chatterjee, Kiran Sankar; Das, Ranabir

Citation:

Citation: Nair, Ashwin; Chatterjee, Kiran Sankar; Jha, Vikram; Das, Ranabir; Shivaprasad, P. "Stability of Begomoviral pathogenicity determinant betaC1 is modulated by mutually antagonistic SUMOylation and SIM interactions" BMC Biol. 18, 110-110 (2020).
PubMed: 32867776

Assembly members:

Assembly members:
Nb-SUMO1, polymer, 114 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: 4100 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Nicotiana benthamiana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-22b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Nb-SUMO1: MHHHHHHLEVLFQGPLGSML QTEEDKKPSGDQAAHINLKV KGQDGNEVFFRIKRSTQLKK LMNAYCDRQSVDFNSIAFLF DGRRLRAEQTPDELEMEDGD EIDAMLHQTGGSTV

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	251
15N chemical shifts	86
1H chemical shifts	86

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Nb-SUMO1 monomer	1

Entities:

Entity 1, Nb-SUMO1 monomer 114 residues - Formula weight is not available

HIS tagged SUMO1 sequence from Nicotiana benthamiana. Residues -16 to -11 represent HIS tag followed by (-10 to 0) enzymatic cleavage site.

1

MET

HIS

LEU

GLU

VAL

2

LEU

PHE

GLN

GLY

PRO

LEU

GLY

SER

MET

LEU

3

GLN

THR

GLU

ASP

LYS

PRO

SER

GLY

4

ASP

GLN

ALA

HIS

ILE

ASN

LEU

LYS

VAL

5

LYS

GLY

GLN

ASP

GLY

ASN

GLU

VAL

PHE

6

ARG

ILE

LYS

ARG

SER

THR

GLN

LEU

LYS

7

LEU

MET

ASN

ALA

TYR

CYS

ASP

ARG

GLN

SER

8

VAL

ASP

PHE

ASN

SER

ILE

ALA

PHE

LEU

PHE

9

ASP

GLY

ARG

LEU

ARG

ALA

GLU

GLN

THR

10

PRO

ASP

GLU

LEU

GLU

MET

GLU

ASP

GLY

ASP

11

GLU

ILE

ASP

ALA

MET

LEU

HIS

GLN

THR

GLY

12

GLY

SER

THR

VAL

Samples:

sample_1: entity_1, [U-100% 13C; U-100% 15N], 1.1 mM

sample_conditions_1: ionic strength: 0.162 M; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Bahrami, Markley, Assadi, and Eghbalnia, Bruker Biospin, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax, Goddard - chemical shift assignment, collection, peak picking, processing

NMR spectrometers:

Bruker Avance 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks