BMRB Entry 50145

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50145

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
1H, 15N, and 13C backbone chemical shift assignments for the first bromodomain of BRD4 (BRD4-BD1)
Deposition date:
2019-12-31
Original release date:
2021-08-12
Authors:
Patel, Karishma; Mackay, Joel; Solomon, Paul
Citation:

Citation: Patel, Karishma; Solomon, Paul; Walshe, James; Ford, Daniel; Wilkinson-White, Lorna; Payne, Richard; Low, Jason; Mackay, Joel. "BET-Family Bromodomains Can Recognize Diacetylated Sequences from Transcription Factors Using a Conserved Mechanism"  Biochemistry 60, 648-662 (2021).
PubMed: 33620209

Assembly members:

Assembly members:
The first bromodomain of BRD4, polymer, 145 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P

Entity Sequences (FASTA):

Entity Sequences (FASTA):
The first bromodomain of BRD4: GPLGSSTNPPPPETSNPNKP KRQTNQLQYLLRVVLKTLWK HQFAWPFQQPVDAVKLNLPD YYKIIKTPMDMGTIKKRLEN NYYWNAQECIQDFNTMFTNC YIYNKPGDDIVLMAEALEKL FLQKINELPTEEPEFPGRLE RPHRD

Data sets:
Data typeCount
13C chemical shifts221
15N chemical shifts105
1H chemical shifts105

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1The first bromodomain of BRD41

Entities:

Entity 1, The first bromodomain of BRD4 145 residues - Formula weight is not available

1   GLYPROLEUGLYSERSERTHRASNPROPRO
2   PROPROGLUTHRSERASNPROASNLYSPRO
3   LYSARGGLNTHRASNGLNLEUGLNTYRLEU
4   LEUARGVALVALLEULYSTHRLEUTRPLYS
5   HISGLNPHEALATRPPROPHEGLNGLNPRO
6   VALASPALAVALLYSLEUASNLEUPROASP
7   TYRTYRLYSILEILELYSTHRPROMETASP
8   METGLYTHRILELYSLYSARGLEUGLUASN
9   ASNTYRTYRTRPASNALAGLNGLUCYSILE
10   GLNASPPHEASNTHRMETPHETHRASNCYS
11   TYRILETYRASNLYSPROGLYASPASPILE
12   VALLEUMETALAGLUALALEUGLULYSLEU
13   PHELEUGLNLYSILEASNGLULEUPROTHR
14   GLUGLUPROGLUPHEPROGLYARGLEUGLU
15   ARGPROHISARGASP

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks