BMRB Entry 50146

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50146

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Title:
1H, 15N, and 13C backbone chemical shift assignments for the second bromodomain of BRD4 (BRD4-BD2)
Deposition date:
2019-12-31
Original release date:
2021-08-12
Authors:
Patel, Karishma; Mackay, Joel; Solomon, Paul
Citation:

Citation: Patel, Karishma; Solomon, Paul; Walshe, James; Ford, Daniel; Wilkinson-White, Lorna; Payne, Richard; Low, Jason; Mackay, Joel. "BET-Family Bromodomains Can Recognize Diacetylated Sequences from Transcription Factors Using a Conserved Mechanism"  Biochemistry 60, 648-662 (2021).
PubMed: 33620209

Assembly members:

Assembly members:
The second bromdomain of BRD4, polymer, 122 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-6P

Entity Sequences (FASTA):

Entity Sequences (FASTA):
The second bromdomain of BRD4: GPLGSSKVSEQLKCCSGILK EMFAKKHAAYAWPFYKPVDV EALGLHDYCDIIKHPMDMST IKSKLEAREYRDAQEFGADV RLMFSNCYKYNPPDHEVVAM ARKLQDVFEMRFAKMPDEPE EP

Data sets:
Data typeCount
13C chemical shifts143
15N chemical shifts84
1H chemical shifts84

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1The second bromodomain of BRD41

Entities:

Entity 1, The second bromodomain of BRD4 122 residues - Formula weight is not available

1   GLYPROLEUGLYSERSERLYSVALSERGLU
2   GLNLEULYSCYSCYSSERGLYILELEULYS
3   GLUMETPHEALALYSLYSHISALAALATYR
4   ALATRPPROPHETYRLYSPROVALASPVAL
5   GLUALALEUGLYLEUHISASPTYRCYSASP
6   ILEILELYSHISPROMETASPMETSERTHR
7   ILELYSSERLYSLEUGLUALAARGGLUTYR
8   ARGASPALAGLNGLUPHEGLYALAASPVAL
9   ARGLEUMETPHESERASNCYSTYRLYSTYR
10   ASNPROPROASPHISGLUVALVALALAMET
11   ALAARGLYSLEUGLNASPVALPHEGLUMET
12   ARGPHEALALYSMETPROASPGLUPROGLU
13   GLUPRO

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks