BMRB Entry 50153
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50153
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Title: Isolation and characterization of antimicrobial peptides with unusual disulfide connectivity from the colonial ascidian Synoicum turgens [Turgencin B(Mox2)] PubMed: 31940927
Deposition date: 2020-01-09 Original release date: 2020-06-09
Authors: Hansen, Ida; Isaksson, Johan; Poth, Aaron; Andersen, Aaron; Hansen, Kine; Richard, Celine; Blencke, Hans-Matti; Stensvaag, Klara; Craik, David; Tor, Haug
Citation: Hansen, Ida; Isaksson, Johan; Poth, Aaron; Hansen, Kine; Andersen, Aaron; Richard, Celine; Blencke, Hans-Matti; Stensvag, Klara; Craik, David; Haug, Tor. "Isolation and characterization of antimicrobial peptides with unusual disulfide connectivity from the colonial ascidian Synoicum turgens" Mar. Drugs 18, .-. (2020).
Assembly members:
Turgencin B (Mox2), polymer, 36 residues, 3575 Da.
Natural source: Common Name: not available Taxonomy ID: 562744 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Synoicum turgens
Experimental source: Production method: purified from the natural source
Entity Sequences (FASTA):
Turgencin B (Mox2): GIKEMLCNMACAQTVCKKSG
GPLCDTCQAACKALGX
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 104 |
15N chemical shifts | 35 |
1H chemical shifts | 223 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | Turgencin B (Mox2) | 1 |
Entities:
Entity 1, Turgencin B (Mox2) 36 residues - 3575 Da.
Methionines are predominantly oxidized
1 | GLY | ILE | LYS | GLU | MET | LEU | CYS | ASN | MET | ALA | ||||
2 | CYS | ALA | GLN | THR | VAL | CYS | LYS | LYS | SER | GLY | ||||
3 | GLY | PRO | LEU | CYS | ASP | THR | CYS | GLN | ALA | ALA | ||||
4 | CYS | LYS | ALA | LEU | GLY | NH2 |
Samples:
sample_1_1: entity_1 2 mg
sample_conditions_1_1: pH: 6; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-1H NOESY | sample_1_1 | isotropic | sample_conditions_1_1 |
2D 1H-15N HSQC | sample_1_1 | isotropic | sample_conditions_1_1 |
2D 1H-1H TOCSY | sample_1_1 | isotropic | sample_conditions_1_1 |
2D 1H-13C HSQC | sample_1_1 | isotropic | sample_conditions_1_1 |
2D 1H-1H COSY | sample_1_1 | isotropic | sample_conditions_1_1 |
2D 1H-13C HMBC | sample_1_1 | isotropic | sample_conditions_1_1 |
2D 1H-13C TOCSYHSQC | sample_1_1 | isotropic | sample_conditions_1_1 |
Software:
X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution
NMR spectrometers:
- Bruker Avance 600 MHz
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CSV: Backbone
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SPARKY: Backbone
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