BMRB Entry 50154
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50154
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Title: C-terminal domain of TDP-43 at acidic pH PubMed: 33417141
Deposition date: 2020-01-09 Original release date: 2021-03-10
Authors: Mompean, Miguel
Citation: Pantoja-Uceda, David; Stuani, Cristiana; Laurents, Douglas; McDermott, Ann; Buratti, Emanuele; Mompean, Miguel. "NMR assignments for the C-terminal domain of human TDP-43" Biomol. NMR Assign. 15, 177-181 (2021).
Assembly members:
TDP-43 267-414, polymer, 169 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pJ411
Entity Sequences (FASTA):
TDP-43 267-414: MGSDKIHHHHHHENLYFQGH
MNRQLERSGRFGGNPGGFGN
QGGFGNSRGGGAGLGNNQGS
NMGGGMNFGAFSINPAMMAA
AQAALQSSWGMMGMLASQQN
QSGPSGNNQNQGNMQREPNQ
AFGSGNNSYSGSNSGAAIGW
GSASNAGSGSGFNGGFGSSM
DSKSSGWGM
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 467 |
| 15N chemical shifts | 163 |
| 1H chemical shifts | 552 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | C-terminal domain of TDP-43 | 1 |
Entities:
Entity 1, C-terminal domain of TDP-43 169 residues - Formula weight is not available
| 1 | MET | GLY | SER | ASP | LYS | ILE | HIS | HIS | HIS | HIS | ||||
| 2 | HIS | HIS | GLU | ASN | LEU | TYR | PHE | GLN | GLY | HIS | ||||
| 3 | MET | ASN | ARG | GLN | LEU | GLU | ARG | SER | GLY | ARG | ||||
| 4 | PHE | GLY | GLY | ASN | PRO | GLY | GLY | PHE | GLY | ASN | ||||
| 5 | GLN | GLY | GLY | PHE | GLY | ASN | SER | ARG | GLY | GLY | ||||
| 6 | GLY | ALA | GLY | LEU | GLY | ASN | ASN | GLN | GLY | SER | ||||
| 7 | ASN | MET | GLY | GLY | GLY | MET | ASN | PHE | GLY | ALA | ||||
| 8 | PHE | SER | ILE | ASN | PRO | ALA | MET | MET | ALA | ALA | ||||
| 9 | ALA | GLN | ALA | ALA | LEU | GLN | SER | SER | TRP | GLY | ||||
| 10 | MET | MET | GLY | MET | LEU | ALA | SER | GLN | GLN | ASN | ||||
| 11 | GLN | SER | GLY | PRO | SER | GLY | ASN | ASN | GLN | ASN | ||||
| 12 | GLN | GLY | ASN | MET | GLN | ARG | GLU | PRO | ASN | GLN | ||||
| 13 | ALA | PHE | GLY | SER | GLY | ASN | ASN | SER | TYR | SER | ||||
| 14 | GLY | SER | ASN | SER | GLY | ALA | ALA | ILE | GLY | TRP | ||||
| 15 | GLY | SER | ALA | SER | ASN | ALA | GLY | SER | GLY | SER | ||||
| 16 | GLY | PHE | ASN | GLY | GLY | PHE | GLY | SER | SER | MET | ||||
| 17 | ASP | SER | LYS | SER | SER | GLY | TRP | GLY | MET |
Samples:
sample_1: entity_1, [U-100% 13C; U-100% 15N], 60-120 uM
sample_conditions_1: pH: 4; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D CC(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D H(NCOCA)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D (H)N(COCA)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNMR, CCPN - chemical shift assignment
NMR spectrometers:
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts