BMRB Entry 50156
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50156
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Title: Backbone assignment of DUSP22 PubMed: 33053837
Deposition date: 2020-01-13 Original release date: 2021-08-12
Authors: Lai, Chih_Hsuan; Chang, Co-Chih; Lyu, Ping-Chiang
Citation: Lai, Chih-Hsuan; Chang, Co-Chih; Chuang, Huai-Chia; Tan, Tse-Hua; Lyu, Ping-Chiang. "Structural Insights into the Active Site Formation of DUSP22 in N-loop-containing Protein Tyrosine Phosphatases" Intern. J. Mol. Sci. 21, 7515-7515 (2020).
Assembly members:
DUSP22, polymer, 157 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21b
Entity Sequences (FASTA):
DUSP22: GPMGNGMNKILPGLYIGNFK
DARDAEQLSKNKVTHILSVH
DSARPMLEGVKYLCIPAADS
PSQNLTRHFKESIKFIHECR
LRGESCLVHCLAGVSRSVTL
VIAYIMTVTDFGWEDALHTV
RAGRSCANPNVGFQRQLQEF
EKHEVHQYRQWLKEEYG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 405 |
| 15N chemical shifts | 139 |
| 1H chemical shifts | 139 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DUSP22 | 1 |
Entities:
Entity 1, DUSP22 157 residues - Formula weight is not available
| 1 | GLY | PRO | MET | GLY | ASN | GLY | MET | ASN | LYS | ILE | ||||
| 2 | LEU | PRO | GLY | LEU | TYR | ILE | GLY | ASN | PHE | LYS | ||||
| 3 | ASP | ALA | ARG | ASP | ALA | GLU | GLN | LEU | SER | LYS | ||||
| 4 | ASN | LYS | VAL | THR | HIS | ILE | LEU | SER | VAL | HIS | ||||
| 5 | ASP | SER | ALA | ARG | PRO | MET | LEU | GLU | GLY | VAL | ||||
| 6 | LYS | TYR | LEU | CYS | ILE | PRO | ALA | ALA | ASP | SER | ||||
| 7 | PRO | SER | GLN | ASN | LEU | THR | ARG | HIS | PHE | LYS | ||||
| 8 | GLU | SER | ILE | LYS | PHE | ILE | HIS | GLU | CYS | ARG | ||||
| 9 | LEU | ARG | GLY | GLU | SER | CYS | LEU | VAL | HIS | CYS | ||||
| 10 | LEU | ALA | GLY | VAL | SER | ARG | SER | VAL | THR | LEU | ||||
| 11 | VAL | ILE | ALA | TYR | ILE | MET | THR | VAL | THR | ASP | ||||
| 12 | PHE | GLY | TRP | GLU | ASP | ALA | LEU | HIS | THR | VAL | ||||
| 13 | ARG | ALA | GLY | ARG | SER | CYS | ALA | ASN | PRO | ASN | ||||
| 14 | VAL | GLY | PHE | GLN | ARG | GLN | LEU | GLN | GLU | PHE | ||||
| 15 | GLU | LYS | HIS | GLU | VAL | HIS | GLN | TYR | ARG | GLN | ||||
| 16 | TRP | LEU | LYS | GLU | GLU | TYR | GLY |
Samples:
sample_1_13C_15N_DUSP22: DUSP22, [13C, 15N], 0.3-1.0 mM; PIPES 40 mM; sodium chloride 100 mM; DSS 0.16 mM
sample_conditions_1_RT: pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1_13C_15N_DUSP22 | isotropic | sample_conditions_1_RT |
| 3D HN(COCA)CB | sample_1_13C_15N_DUSP22 | isotropic | sample_conditions_1_RT |
| 3D HNCACB | sample_1_13C_15N_DUSP22 | isotropic | sample_conditions_1_RT |
| 3D HNCA | sample_1_13C_15N_DUSP22 | isotropic | sample_conditions_1_RT |
| 3D HN(CO)CA | sample_1_13C_15N_DUSP22 | isotropic | sample_conditions_1_RT |
| 3D HNCO | sample_1_13C_15N_DUSP22 | isotropic | sample_conditions_1_RT |
Software:
TOPSPIN, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - chemical shift assignment
NMR spectrometers:
- Bruker AMX 850 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts