BMRB Entry 50160

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50160

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Title:
Point mutant of Oligosaccharyltransferase subunit, Ost4V23D
Deposition date:
2020-01-16
Original release date:
2020-05-30
Authors:
Chaudhary, Bharat; Zoetewey, David; Mohanty, Smita
Citation:

Citation: Chaudhary, Bharat; Zoetewey, David; Mohanty, Smita. "1H, 13C, 15N Resonance Assignments and Secondary Structure of Yeast Oligosaccharyltransferase Subunit Ost4 and Its Functionally Important Mutant Ost4V23D"  Biomelecular NMR Assignments 14, 205-209 (2020).
PubMed: 32328881

Assembly members:

Assembly members:
Ost4V23D, polymer, 45 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: Pet23a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ost4V23D: MISDEQLNSLAITFGIVMMT LIDIYHAVDSTMSPKNRLEH HHHHH

Data sets:
Data typeCount
13C chemical shifts82
15N chemical shifts39
1H chemical shifts245

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Point mutant Ost4V23D1

Entities:

Entity 1, Point mutant Ost4V23D 45 residues - Formula weight is not available

1   METILESERASPGLUGLNLEUASNSERLEU
2   ALAILETHRPHEGLYILEVALMETMETTHR
3   LEUILEASPILETYRHISALAVALASPSER
4   THRMETSERPROLYSASNARGLEUGLUHIS
5   HISHISHISHISHIS

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