BMRB Entry 50175

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50175

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Title:
Rab1b bound to GDP (AMPylation at Y77)
Deposition date:
2020-01-28
Original release date:
2020-05-30
Authors:
Kang, Hyun-Seo; Barthelmes, Katja; Sattler, Michael
Citation:

Citation: Barthelmes, Katja; Ramcke, Evelyn; Kang, Hyun-Seo; Sattler, Michael; Itzen, Aymelt. "Conformational Control of Small GTPases by AMPylation"  Proc. Natl. Acad. Sci. U.S.A. 117, 5772-5781 (2020).
PubMed: 32123090

Assembly members:

Assembly members:
entity_1_Rab1b, polymer, 173 residues, Formula weight is not available
entity_GDP, non-polymer, 443.201 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMAL

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1_Rab1b: MPEYDYLFKLLLIGDSGVGK SCLLLRFADDTYTESYISTI GVDFKIRTIELDGKTIKLQI WDTAGAERFRTITSSYYRGA HGIIVVYDVTDQESYANVKQ WLQEIDRYASENVNKLLVGN KSDLTTKKVVDNTTAKEFAD SLGIPFLETSAKNATNVEQA FMTMAAEIKKRMG

Data sets:
Data typeCount
15N chemical shifts144
1H chemical shifts155

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Rab1b1
2GDP2

Entities:

Entity 1, Rab1b 173 residues - Formula weight is not available

AMPylation at Y77

1   METPROGLUTYRASPTYRLEUPHELYSLEU
2   LEULEUILEGLYASPSERGLYVALGLYLYS
3   SERCYSLEULEULEUARGPHEALAASPASP
4   THRTYRTHRGLUSERTYRILESERTHRILE
5   GLYVALASPPHELYSILEARGTHRILEGLU
6   LEUASPGLYLYSTHRILELYSLEUGLNILE
7   TRPASPTHRALAGLYALAGLUARGPHEARG
8   THRILETHRSERSERTYRTYRARGGLYALA
9   HISGLYILEILEVALVALTYRASPVALTHR
10   ASPGLNGLUSERTYRALAASNVALLYSGLN
11   TRPLEUGLNGLUILEASPARGTYRALASER
12   GLUASNVALASNLYSLEULEUVALGLYASN
13   LYSSERASPLEUTHRTHRLYSLYSVALVAL
14   ASPASNTHRTHRALALYSGLUPHEALAASP
15   SERLEUGLYILEPROPHELEUGLUTHRSER
16   ALALYSASNALATHRASNVALGLUGLNALA
17   PHEMETTHRMETALAALAGLUILELYSLYS
18   ARGMETGLY

Entity 2, GDP - C10 H15 N5 O11 P2 - 443.201 Da.

1   GDP

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