BMRB Entry 50186

Name: Identification of a novel N-linked glycan on the Archaellins and S-layer protein of the thermophilic methanogen, Methanothermococcus thermolithotrophicus
Published: 2020-08-06

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50186

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title: Identification of a novel N-linked glycan on the Archaellins and S-layer protein of the thermophilic methanogen, Methanothermococcus thermolithotrophicus PubMed: 32817340

Deposition date: 2020-01-30 Original release date: 2020-08-06

Authors: Kelly, John; Vinogradov, Evguenii; Stupak, Jacek; Robotham, Anna; Logan, Susan; Berezuk, Alison; Khursigara, Cezar; Jarrell, Ken

Citation: Kelly, John; Vinogradov, Evgeny; Stupak, Jacek; Robotham, Anna; Logan, Susan; Berezuk, Alison; Khursigara, Cezar; Jarrell, Ken. "Identification of a novel N-linked glycan on the archaellins and S-layer protein of the thermophilic methanogen, Methanothermococcus thermolithotrophicus" J. Biol. Chem. 295, 14618-14629 (2020).

Assembly members:
entity_1, polymer, 7 residues, Formula weight is not available

Natural source: Common Name: Methanothermococcus thermolithotrophicus Taxonomy ID: 2186 Superkingdom: Archaea Kingdom: not available Genus/species: Methanothermococcus thermolithotrophicus

Experimental source: Production method: purified from the natural source

Entity Sequences (FASTA):
entity_1: XXXXXXX

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	44
1H chemical shifts	42

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	heptameric archaellin N-glycan	1

Entities:

Entity 1, heptameric archaellin N-glycan 7 residues - Formula weight is not available

1

XA

XB

XC

XD

XE

XF

XG

Samples:

sample_1: entity_1 1 mM

sample_conditions_1: temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.2, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 600 MHz