BMRB Entry 50191
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50191
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Title: RBM5 RRM1-Zf1-C191G PubMed: 32450081
Deposition date: 2020-02-04 Original release date: 2020-06-09
Authors: Soni, Komal; Sattler, Michael
Citation: Soni, Komal; Martinez-Lumbreras, Santiago; Sattler, Michael. "Conformational Dynamics From Ambiguous Zinc Coordination in the RanBP2-Type Zinc Finger of RBM5" J. Mol. Biol. 432, 4127-4138 (2020).
Assembly members:
entity_1, polymer, . residues, Formula weight is not available
entity_ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
entity_1: MGERESKTIMLRGLPTTITE
SDIREMMESFEGPQPADVRL
MKRKTGVSRGFAFVEFYHLQ
DATSWMEANQKKLVIQGKHI
AMHYSNPRPKFEDWLCNKCG
LNNFRKRLKCFRCGADKFD
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 106 |
| 1H chemical shifts | 106 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RRM1-Zf1 | 1 |
| 2 | Zn | 2 |
Entities:
Entity 1, RRM1-Zf1 - Formula weight is not available
Sequence numbering starts from 92 and residues 92, 93 are cloning artifacts.
| 1 | MET | GLY | GLU | ARG | GLU | SER | LYS | THR | ILE | MET | ||||
| 2 | LEU | ARG | GLY | LEU | PRO | THR | THR | ILE | THR | GLU | ||||
| 3 | SER | ASP | ILE | ARG | GLU | MET | MET | GLU | SER | PHE | ||||
| 4 | GLU | GLY | PRO | GLN | PRO | ALA | ASP | VAL | ARG | LEU | ||||
| 5 | MET | LYS | ARG | LYS | THR | GLY | VAL | SER | ARG | GLY | ||||
| 6 | PHE | ALA | PHE | VAL | GLU | PHE | TYR | HIS | LEU | GLN | ||||
| 7 | ASP | ALA | THR | SER | TRP | MET | GLU | ALA | ASN | GLN | ||||
| 8 | LYS | LYS | LEU | VAL | ILE | GLN | GLY | LYS | HIS | ILE | ||||
| 9 | ALA | MET | HIS | TYR | SER | ASN | PRO | ARG | PRO | LYS | ||||
| 10 | PHE | GLU | ASP | TRP | LEU | CYS | ASN | LYS | CYS | GLY | ||||
| 11 | LEU | ASN | ASN | PHE | ARG | LYS | ARG | LEU | LYS | CYS | ||||
| 12 | PHE | ARG | CYS | GLY | ALA | ASP | LYS | PHE | ASP |
Entity 2, Zn - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: entity_1, [U-15], mM; MES 20 mM; sodium chloride 400 mM; DTT 1 mM
sample_conditions_1: ionic strength: 0.4 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
Analysis, CCPN - chemical shift assignment
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 600 MHz
Related Database Links:
| NCBI | NP_005769.1 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts