BMRB Entry 50201
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50201
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Title: Chemical shift assignment of the Connexin43 cytoplasmic loop domain PubMed: 33080786
Deposition date: 2020-02-20 Original release date: 2021-08-11
Authors: Spagnol, Gaelle; Chenavas, Sylvie; Kieken, Fabien; Trease, Andrew; Zheng, Li; Brownell, Sarah; Anbanandam, Asokan; Sorgen, Paul
Citation: Zheng, Li; Chenavas, Sylvie; Kieken, Fabien; Trease, Andrew; Brownell, Sarah; Anbanandam, Asokan; Sorgen, Paul; Spagnol, Gaelle. "Calmodulin Directly Interacts with the Cx43 Carboxyl-Terminus and Cytoplasmic Loop Containing Three ODDD-Linked Mutants (M147T, R148Q, and T154A) that Retain \u03b1-Helical Structure, but Exhibit Loss-of-Function and Cellular Trafficking Defects" Biomolecules 10, 1452-1452 (2020).
Assembly members:
entity_1, polymer, 61 residues, 7071.2 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX6p2
Entity Sequences (FASTA):
entity_1: GPLGSMRKEEKLNKKEEELK
VAQTDGVNVEMHLKQIEIKK
FKYGIEEHGKVKMRGGLLRT
Y
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 259 |
| 15N chemical shifts | 59 |
| 1H chemical shifts | 448 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Cytoplasmic loop domain of connexin43 | 1 |
Entities:
Entity 1, Cytoplasmic loop domain of connexin43 61 residues - 7071.2 Da.
Residues 1-5 represent part of the remaining non-native affinity tag. This is the cytoplasmic loop domain (residues 100-155) of a 4-span transmembrane protein.
| 1 | GLY | PRO | LEU | GLY | SER | MET | ARG | LYS | GLU | GLU | ||||
| 2 | LYS | LEU | ASN | LYS | LYS | GLU | GLU | GLU | LEU | LYS | ||||
| 3 | VAL | ALA | GLN | THR | ASP | GLY | VAL | ASN | VAL | GLU | ||||
| 4 | MET | HIS | LEU | LYS | GLN | ILE | GLU | ILE | LYS | LYS | ||||
| 5 | PHE | LYS | TYR | GLY | ILE | GLU | GLU | HIS | GLY | LYS | ||||
| 6 | VAL | LYS | MET | ARG | GLY | GLY | LEU | LEU | ARG | THR | ||||
| 7 | TYR |
Samples:
sample_1: Cx43CL domain, [U-99% 13C; U-99% 15N], 1 mM; sodium phosphate 10 mM; potassium phosphate 1.8 mM; potassium chloride 2.7 mM; sodium chloride 137 mM
sample_conditions_1: ionic strength: 0.154 M; pH: 5.8; pressure: 1 atm; temperature: 280 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNHA | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(COCA)CB | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRView v5.2.2.01 - chemical shift assignment, data analysis, peak picking
NMRPipe - processing
NMR spectrometers:
- Varian INOVA 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts