BMRB Entry 50214
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50214
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Title: 1HN and 15N assignment of PhoQ PD at pH 6.5 PubMed: 26002083
Deposition date: 2020-03-18 Original release date: 2020-04-21
Authors: Hicks, Kevin; Scott, Delbecq; Enea, Sancho-Vaello; Marie-Pierre, Blanc; Katja, Dove; Lynne, Prost; Margaret, Daley; Kornelius, Zeth; Rachel, Klevit; Samuel, Miller
Citation: Hicks, Kevin; Delbecq, Scott; Sancho-Vaello, Enea; Blanc, Marie-Pierre; Dove, Katja; Prost, Lynne; Daley, Margaret; Zeth, Kornelius; Klevit, Rachel; Miller, Samuel. "Acidic pH and divalent cation sensing by PhoQ are dispensable for systemic salmonellae virulence." Elife 4, e06792-e06792 (2015).
Assembly members:
entity_1, polymer, 153 residues, Formula weight is not available
Natural source: Common Name: Salmonella typhimurium Taxonomy ID: 28901 Superkingdom: Bacteria Kingdom: not available Genus/species: Salmonella enterica
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET11a-phoQ 45-190-(His)6
Entity Sequences (FASTA):
entity_1: MDKTTFRLLRGESNLFYTLA
KWENNKISVELPENLDMQSP
TMTLIYDETGKLLWTQRNIP
WLIKSIQPEWLKTNGFHEIE
TNVDATSTLLSEDHSAQEKL
KEVREDDDDAEMTHSVAVNI
YPATARMPQLTIVVVDTIPI
ELKRSYMHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 125 |
| 1H chemical shifts | 125 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | PhoQ_PD | 1 |
Entities:
Entity 1, PhoQ_PD 153 residues - Formula weight is not available
| 1 | MET | ASP | LYS | THR | THR | PHE | ARG | LEU | LEU | ARG | ||||
| 2 | GLY | GLU | SER | ASN | LEU | PHE | TYR | THR | LEU | ALA | ||||
| 3 | LYS | TRP | GLU | ASN | ASN | LYS | ILE | SER | VAL | GLU | ||||
| 4 | LEU | PRO | GLU | ASN | LEU | ASP | MET | GLN | SER | PRO | ||||
| 5 | THR | MET | THR | LEU | ILE | TYR | ASP | GLU | THR | GLY | ||||
| 6 | LYS | LEU | LEU | TRP | THR | GLN | ARG | ASN | ILE | PRO | ||||
| 7 | TRP | LEU | ILE | LYS | SER | ILE | GLN | PRO | GLU | TRP | ||||
| 8 | LEU | LYS | THR | ASN | GLY | PHE | HIS | GLU | ILE | GLU | ||||
| 9 | THR | ASN | VAL | ASP | ALA | THR | SER | THR | LEU | LEU | ||||
| 10 | SER | GLU | ASP | HIS | SER | ALA | GLN | GLU | LYS | LEU | ||||
| 11 | LYS | GLU | VAL | ARG | GLU | ASP | ASP | ASP | ASP | ALA | ||||
| 12 | GLU | MET | THR | HIS | SER | VAL | ALA | VAL | ASN | ILE | ||||
| 13 | TYR | PRO | ALA | THR | ALA | ARG | MET | PRO | GLN | LEU | ||||
| 14 | THR | ILE | VAL | VAL | VAL | ASP | THR | ILE | PRO | ILE | ||||
| 15 | GLU | LEU | LYS | ARG | SER | TYR | MET | HIS | HIS | HIS | ||||
| 16 | HIS | HIS | HIS |
Samples:
sample_1: PhoQ_PD, [U-100% 15N], 400 uM; sodium phosphate 20 mM; sodium chloride 150 mM; magnesium chloride 20 mM; EDTA 20 mM
sample_conditions_1: ionic strength: 0.2 M; pH: 6.5; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNmr Analysis - data analysis
NMR spectrometers:
- Bruker Avance 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts